This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful
Methods in Molecular Biology
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge,
Protein Engineering: Methods and Protocols
aims to aid scientists in the planning and performance of their experiments.
The chapter 'Functional Analysis of Membrane Proteins Produced by Cell-Free Translation' is open access under a CC BY 4.0 license via link.springer.com.
Reihe
Auflage
Sprache
Verlagsort
Verlagsgruppe
Illustrationen
19 s/w Abbildungen, 63 farbige Abbildungen
XI, 350 p. 82 illus., 63 illus. in color.
ISBN-13
978-1-4939-7366-8 (9781493973668)
DOI
10.1007/978-1-4939-7366-8
Schweitzer Klassifikation