Collection of Untargeted Metabolomic Data for Mammalian Urine Applying HILIC and Reversed Phase Ultra Performance Liquid Chromatography Methods Coupled to a Q Exactive Mass Spectrometer.- Isotopic Ratio Outlier Analysis (IROA) for Quantitative Analysis.- Nuclear Magnetic Resonance Measurement of Metabolic Flux Using 13C and 1H Signals.- Quantitative Metabolomics using Isotope Residue Outlier Analysis (IROA®) with Internal Standards.- Analyses of Cholesterol Metabolites of Optic Nerve using GC-MS Methods.- Analyses of Cholesterol and Derivatives in Ocular Tissues using LC-MS/MS Methods.- Measurement of Pyridine Nucleotides in Biological Samples using LC-MS/MS.- Handling and Analysis of 5-Formyl-, 5,10-Methenyl-, 10-Formyl-, 5-Formimno-, and 5,10-Methylenetetrahydrofolates.- Protein-Lipid Complex Separation Utilizing a Capillary Electrophoresis System.- Immature and Mature Collagen Crosslinks Quantification using High Performance Liquid Chromatography and High Resolution Mass Spectrometry in OrbitrapTM.- Relative Quantitation of Neurotoxic Metabolites of the Kynurenine Pathway by Laser Desorption Ionization Mass Spectrometry (LDI-MS).- Analysis of Microbial Siderophores by Mass Spectrometry.- HSD18B7 Enzyme Assay Technique using a Triple Quadrupole Mass Spectrometer.- Assays for Intracellular Cyclic Adenosine Monophosphate (cAMP) and Lysosomal Acidification.- Isotopic Ratio Outlier Analysis (IROA) of Aqueous Humor for Metabolites.- Assessment of Transport of Lipid Metabolites within Trabecular Meshwork Cells.- Detection and Quantification of Lipid Droplets in Differentiated Human Podocytes.- Labeling Cancer Stem Cells with 13C6 Glucose and 13C5 Glutamine for Metabolic Flux Analysis.- Protocols for Metabolomic Analyses from Tissues in Parkinson's Disease.- Application of Stable Isotope Labels for Metabolomics in Studies in Fatty Liver Disease.- Proteometabolomics ofMelphalan Resistance in Multiple Myeloma.- Metabolomic Analyses of Muscle Atrophy Induced by Hindlimb Unloading.- Protocols for NMR Analysis in Livestock Metabolomics.- Metabolomics Applicable to Retinal Vascular Diseases.