This detailed volume provides a comprehensive set of experimental protocols and useful strategies to examine the repair of damaged bases via the Base Excision Repair (BER) pathway
in vitro
and in cells. Beginning with multiple molecular and cellular techniques to examine the excision of damaged bases from double-stranded DNA or DNA wrapped in a nucleosome, the book continues with sections covering procedures to detect and quantify the damaged bases, protein DNA crosslinks, and double-strand breaks, experimental procedures to identify DNA repair protein interactome by conventional tandem affinity purification followed by mass spectroscopy analysis, as well as the analysis of genome-wide binding of DNA repair proteins and copy number variations of the DNA damage response gene in tumors. Written for the highly successful
Methods in Molecular Biology
series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical,
Base Excision Repair Pathway: Methods and Protocols
serves as a valuable resource for novices and experts trying to examine the repair of various types of DNA lesions
in vitro
and in cell by the distinct set of proteins in the BER pathway.
Reihe
Sprache
Verlagsort
Verlagsgruppe
Illustrationen
35 farbige Abbildungen, 12 s/w Abbildungen
XIII, 262 p. 47 illus., 35 illus. in color.
Dateigröße
ISBN-13
978-1-0716-3373-1 (9781071633731)
DOI
10.1007/978-1-0716-3373-1
Schweitzer Klassifikation
