Immunocytochemistry and in situ hybridization are widely used biomedical
sciences. They are essential in medical diagnosis and in cell biology
research. Affinity labeling is the central goal of the experimental
strategy involving a series of techniques in a logical order; from the
effects of specimen fixation, through specimen preparation to expose the
antigen, to optimizing immunolabeling, to assessing the result and
finally to safety considerations. Numerous examples of these techniques
in biomedical sciences are included, as well as experimental assays and
practical tips. This survey of methods will serve as an invaluable
reference source in any laboratory setting (academic, industrial or
clinical) involved in research in almost every branch of biology or
medicine, as well as in pharmaceutical, biotechnological and clinical
applications.
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Dateigröße
ISBN-13
978-1-4612-0139-7 (9781461201397)
DOI
10.1007/978-1-4612-0139-7
Schweitzer Klassifikation
1 Introduction.- 2 Preservation of Tissue for Immunocytochemical Studies.- 3 Specimen Preparation for Immunocytochemistry.- 4 Antigen Retrieval.- 5 Immunolabeling.- 6 In Situ Hybridization: General Principles and Application of Digoxigenin-Labeled cRNA for the Detection of mRNAs.- 7 Fluorescence In Situ Hybridization.- 8 In Situ Amplification and Detection of Nucleic Acids.- 9 Image Analysis and Statistics: A Quantitative Approach to Immunocytochemistry and Related Techniques.- 10 Safety in the Laboratory.- 11 Applications of Affinity Labeling in Biomedical Sciences.
