Handbook of Histology Methods for Bone and Cartilage

Part I. Introduction

Introduction to Experimental Bone and Cartilage Histology
Yuehuei H. An and Helen E. Gruber

Part II. Structure and Function of Bone and Cartilage

Cell Structure and Biology of Bone and Cartilage
William R. Walsh, Mark Walton, Warwick Bruce, Yan Yu, Ronald M. Gillies, and Martin Svehla

Normal Structure and Function of Bone
Torben Steiniche and Ellen M. Hauge

Structure and Function of Articular Cartilage
Jerry C. Y. Hu and Kyriacos A. Athanasiou

Part III. Tissue Harvesting, Fixation, and Preparation

Bone-Labeling Techniques
Reinhold G. Erben

Human Bone Biopsy
Robert S. Weinstein

Biopsy Issues for Bone and Cartilage Tumors
John L. Eady

Tissue Harvesting and Fixation
Linda L. Jenkins and Karen J. L. Burg

Common Fixatives in Hard-Tissue Histology
Antonio Scarano, Giovanna Iezzi, and Adriano Piattelli

Decalcification of Bone Tissue
Robert A. Skinner

Principles of Embedding and Common Protocols
Yuehuei H. An, Patricia L. Moreira, Qian K. Kang, and Helen E. Gruber

Infiltration Techniques and Results in Different Types of Resin
Antonio Scarano, Giovanna Orsini, and Adriano Piattelli

Part IV. Sectioning Techniques

Histological Techniques for Decalcified Bone and Cartilage
Qian K. Kang, Jackie C. LaBreck, Helen E. Gruber, and Yuehuei H. An

Techniques for Sectioning Undecalcified Bone Tissue Using Microtomes
William L. Ries

Cutting and Grinding Methods for Hard-Tissue Histology
Thomas W. Bauer and Diane Mahovlic

Bone Sectioning Using the Exakt System
Karl Donath and Michael Rohrer

Bone Sectioning Using a Modified Inner Diamond Saw
Joop G. C. Wolke, Jan-Paul C. M. van der Waerden, Christel P. A. T. Klein, and John A. Jansen

Bone Sectioning Using the Precise 1 Automated Cutting System
Antonio Scarano, Manlio Quaranta, and Adriano Piattelli

Bone, Cement, and Metal Implant Interface Preparation with a High-Pressure Water Cutter
Jian-Sheng Wang and Lars Lidgren

Part V. Staining Techniques

Basic Staining and Histochemical Techniques and Immunohistochemical Localizations Using Bone Sections
Helen E. Gruber and Jane A. Ingram

Basic Staining Techniques for Cartilage Sections
Helen E. Gruber and Jane A. Ingram

Histochemical and Immunohistochemical Staining of Cartilage Sections
Andreas G. Nerlich

Staining Techniques for Plastic-Embedded Specimens
Antonio Scarano, Giovanna Petrone, and Adriano Piattelli

In Situ Hybridization of Bone and Cartilage
Shintaro Nomura and Seiichi Hirota

Part VI. Analysis Techniques

Bone Histomorphometry: Concepts and Common Techniques
Jean E. Aaron and Patricia A. Shore

Histological Analysis of Bone-Implant Interface
John A. Jansen

Histomorphometric Analysis of Bone-Cement and Cement-Metal Interface
Jian-Sheng Wang, Gonzalo G. Valdivia, Michael J. Dunbar, Cecil H. Rorabeck, Robert B. Bourne, and Suzanne Maher

Histologic Analysis of Bone Healing
Ryland B. Edwards, III, Mandi J. Lopez, and Mark D. Markel

Histomorphometry of Metabolic Bone Conditions
Ellen M. Hauge, Torben Steiniche, and Troels T. Andreassen

Histology of Articular Cartilage Repair
Stefan Nehrer and Myron Spector

Histological Analysis of Cartilage Conditions
Theodore R. Oegema, Jr., Cathy S. Carlson, and Ada A. Cole

Histological Analysis of Soft-Tissues Biomaterial Interface: Relevance to Dental Implants
Antonio Scarano, Gian Antonio Favero, Elisabetta Fiera, and Adriano Piattelli

Pathological Diagnosis of Common Tumors of Bone and Cartilage
Jasvir S. Khurana and Kri