Redox Chemistry and Biology of Thiols offers an applied, comprehensive overview of redox chemistry and biology of thiol-dependent processes. Running from basic biology and chemistry of redox phenomena to research methods and highlighting recently identified roles of thiols across cellular and bodily systems, this book draws upon a range of disciplines to illuminate new research directions, new applications of thiol studies, and clinical translation. Sections cover thiol oxidizing species, thiol reactivity and modifications, thioredoxin, glutaredoxin, glutathione, peroxidases, thiol repair enzymes, thiol oxidative signaling, disulfide bond formation, thiol-based redox biosensors, cysteine and hydrogen sulfide metabolism, iron-sulfur cluster biogenesis, thiols in chloroplasts, blood thiols, sugar and polyamine thiols in pathogenic organisms, redox medicine (therapeutic applications of thiols and drug development), as well as methods and bioinformatics tools.
- Runs from basic thiol biology and chemistry to applications and clinical translation
- Provides methods and protocols that will power new research across biomedicine, cell biology, plant biology, drug development, and protein folding and modulation
- Includes chapter contributions from international leaders in the field
Sprache
Verlagsort
Verlagsgruppe
Elsevier Science & Techn.
Illustrationen
Approx. 250 illustrations (150 in full color)
Dateigröße
ISBN-13
978-0-323-91566-3 (9780323915663)
Schweitzer Klassifikation
1. Basic concepts of thiol chemistry and biology2. Chemical basis of cysteine reactivity: Acidity and nucleophilicity3. Computational functional analysis of cysteine residues in proteins4. Global approaches for protein thiol redox state detection and quantification5. Thiol oxidation by biologically-relevant reactive species6. Thiyl radicals: formation, properties and detection7. Detection of the oxidation products of thiols: disulfides, sulfenic, sulfinic and sulfonic acids8. Biochemistry and detection of S-nitrosothiols9. Thiol modification and signaling by biological electrophiles10. Thioredoxin and glutathione reductases11. Functional plasticity in the thioredoxin family: FeS-thio- and glutaredoxins12. Glutathione and glutathione-dependent enzymes13. Thiol and selenol-based peroxidases: Structure and catalytic properties 14. Thiol peroxidase-based redox relays 15. Compartmentalized disulphide bond formation pathways 16. Disulfide bond formation in Escherichia coli17. Thiol-based redox probes18. Selenocysteine-containing proteins19. Overview of cysteine metabolism20. Hydrogen sulfide and persulfides21. The role of thiols in iron-sulfur cluster biogenesis22. Thiol-based redox control in chloroplasts23. Sugar-based cysteine thiols recruited for oxidative stress defence and redox regulation24. Polyamine-based thiols in pathogens25. Thiols in blood26. A thiol chemistry perspective on redox medicine27. Therapeutic applications of low-molecular-weight thiols and selenocompounds28. Thiol targets in drug development to combat bacterial infections
