Since the publication of the first edition, the techniques and experimental approaches employed in the study of adipose tissue have undergone radical expansion and revision. In Adipose Tissue Protocols: Second Edition, several updated chapters are combined with many new contributions from internationally recognized experts covering both conventional and state-of-the-art methodologies as well as whole animal, cellular and molecular approaches which have emerged in or been extended to adipose tissue research over the past few years. As a volume in the highly successful Methods in Molecular BiologyT series, each chapter includes an introduction to its subject matter, a list of necessary materials, step-by-step, readily reproducible laboratory protocols, and a Notes section, which provides invaluable tips and tricks to safeguard against potential mishaps and pitfalls.
Cutting-edge and comprehensive, Adipose Tissue Protocols: Second Edition is an essential manual for newcomers tothis burgeoning area as well as a valuable addition to the laboratories and offices of more experienced researchers.
Reihe
Auflage
Softcover reprint of hardcover 2nd ed. 2008
Sprache
Verlagsort
Zielgruppe
Für Beruf und Forschung
Research
Editions-Typ
Illustrationen
Maße
Höhe: 235 mm
Breite: 155 mm
Dicke: 22 mm
Gewicht
ISBN-13
978-1-61737-844-7 (9781617378447)
DOI
10.1007/978-1-59745-245-8
Schweitzer Klassifikation
Overview of Adipose Tissue and Its Role in Obesity and Metabolic Disorders.- Choosing an Adipose Tissue Depot for Sampling.- Application of Imaging and Other Noninvasive Techniques in Determining Adipose Tissue Mass.- Generation of Adipose Tissue-Specific Transgenic Mouse Models.- Angiogenesis in Adipose Tissue.- Adipose Organ Nerves Revealed by Immunohistochemistry#.- Human Adipose Tissue Blood Flow and Micromanipulation of Human Subcutaneous Blood Flow.- Studies of Thermogenesis and Mitochondrial Function in Adipose Tissues.- Application of Lipidomics and Metabolomics to the Study of Adipose Tissue.- Applications of Proteomics to the Study of Adipose Tissue.- Application of DNA Microarray to the Study of Human Adipose Tissue/Cells.- Differentiation of Adipose Stem Cells.- Methods That Resolve Different Contributions of Clonal Expansion to Adipogenesis in 3T3-L1 and C3H10T1/2 Cells.- Explant Cultures of White Adipose Tissue.- Isolation and Culture of Preadipocytes from Rodent White Adipose Tissue.- Flow Cytometry on the Stromal-Vascular Fraction of White Adipose Tissue.- Application of Electrophoretic Mobility Shift Assay and Chromatin Immunoprecipitation in the Study of Transcription in Adipose Cells.- Application of RNA Interference Techniques to Adipose Cell Cultures.- RNA Isolation and Real-Time Quantitative RT-PCR.- Study of Adipose Tissue Gene Expression by In Situ Hybridization.- Application of Immunocytochemistry and Immunofluorescence Techniques to Adipose Tissue and Cell Cultures.- Determination of Lipolysis in Isolated Primary Adipocytes.- Study of Glucose Uptake in Adipose Cells.- Measurement of Phosphoinositide 3-Kinase and Its Products to Study Adipogenic Signal Transduction.
