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This volume details methods for the analyses of specific lipid classes and lipidomics analyses of cells such as lymphocytes and oocytes. Lipidomics guides readers through chapters on direct-flow and chromatographic methods (SFC, UHPLC, HPTLC, ion-mobility); derivatization methods for lipids (amines, fatty aldehydes and ketones); TOF-SIMS imaging of lipids; and characterization of lipid transfer proteins. Additional chapters also provide an authoritative overview of lipidomics strategies and a detailed review of high-resolution mass spectrometric methods are included in this volume. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory. Concise and easy-to-use, Lipidomics aims to ensure successful results in the further study of this vital field.
Introduction and Overview of Lipidomic Strategies.- Non-targeted Lipidomics Utilizing Constant Infusion High Resolution ESI Mass Spectrometry.- Ultrahigh-Resolution Lipid Analysis with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry.- MALDI-Imaging Mass Spectrometry of Brain Lipids.- Ion-Mobility Mass Spectrometry for Lipidomics Applications.- In vitro Assay to Extract Specific Lipid Types from Phospholipid Membranes using Lipid-Transfer Proteins: A Lesson from the Ceramide Transport Protein CERT.- Quantification of Endogenous Endocannabinoids by LC-MS/MS.- Lipid Profiling by Supercritical Fluid Chromatography/Mass Spectrometry.- Mass Spectrometric Analysis of Lipid Hydroperoxides.- Mass Spectrometric Determination of Fatty Aldehydes Exemplified by Monitoring the Oxidative Degradation of (2E)-hexadecenal in HepG2 Cell Lysates.- CE Analysis of Phospholipid Headgroups.- HPTLC-MALDI TOF MS Imaging Analysis of Phospholipids.- Global UHPLC/HRMS Lipidomics Workflow for the Analysis of Lymphocyte Suspension Cultures.- Ambient Lipidomic Analysis of Brain Tissue using Desorption Electrospray Ionization (DESI) Mass Spectrometry.- Lipidomics Analyses of Oxygenated Metabolites of Polyunsaturated Fatty Acids.- Derivatization of Fatty Aldehydes and Ketones: Girard's Reagent T (GRT).- Derivatization of Lipid Amines: Fluorenylmethyloxycarbonyl (FMOC).- High-Resolution Mass Spectrometry of Glycerophospholipid Oxidation Products.
