Biocatalysts are increasingly used by chemists engaged in fine chemical synthesis within both industry and academia. Today, there exists a huge choice of high-tech enzymes and whole cell biocatalysts, which add enormously to the repertoire of synthetic possibilities.
Practical Methods for Biocatalysis and Biotransformations is a "how-to" guide focussing on commercially available enzymes and strains of microorganisms that are readily obtained from culture collections. The source of starting materials and reagents, hints, tips and safety advice (where appropriate) are given to ensure, as far as possible, that the procedures are reproducible. Comparisons to alternative methodology are given and relevant references to the primary literature are cited. Contents include:
* Introduction to the application of biocatalysts in the pharma industry
* introduction to molecular biological techniques for biocatalyst development
* Lipase and protease methods including enzyme supports and dynamic kinetic resolutions
* Nitrile hydrolases and hydratases
* Alcohol dehydrogenases and co-factor recycling
* Epoxide hydrolases
* Amino acid synthesis
* Whole cell processes
* Other biocatalysts, including monoamineoxidases, aminotransterases, and lyases
Practical Methods for Biocatalysis and Biotransformations is an essential text offering validated biocatalyst methods for researchers and postgraduate students in industry and academia.
Rezensionen / Stimmen
"Chapters cover key biotransformations from the literature, detailing the procedures used, the sources of the starting materials and reagents, references, and tips and advice, where appropriate." (JACS, 2010) "It starts with a review of the current use of biocatalysis in the pharmaceutical industry. Although this is focused on a large pharmaceutical company (GlaxoSmithKline), it covers a wide variety of aspects of the industry and offers insightful comparisons of biocatalysis with 'traditional' organic synthesis." (Chemistry World, July 2010)
"I can recommend to all those who are interesting in carrying out biotransformations in the laboratory, and the book should help persuade those who have never used a biotransformation in an organic synthesis, to try one for the first time." (Organic Process Research & Development Journal, 2010)
Produkt-Info
Auflage
Sprache
Verlagsort
Zielgruppe
Produkt-Hinweis
Maße
Höhe: 249 mm
Breite: 173 mm
Dicke: 28 mm
Gewicht
ISBN-13
978-0-470-51927-1 (9780470519271)
Schweitzer Klassifikation
John Whittall
CoEBio3, Manchester Interdisciplinary Biocentre, Manchester University, UK
Peter W Sutton
GlaxoSmithKline Research and Development Limited, UK
Autor*in
University of Manchester
GlaxoSmithKline R&D Ltd
Preface.
Abbreviations.
List of Contributors.
1 Biotransformations in Small-molecule Pharmaceutical Development (Joseph P. Adams, Andrew J. Collis, Richard K. Henderson and Peter W. Sutton).
2 Biocatalyst Identification and Scale-up: Molecular Biology for Chemists (Kathleen H. McClean).
3 Kinetic Resolutions Using Biotransformations.
4 Dynamic Kinetic Resolution for the Synthesis of Esters, Amides and Acids Using Lipases.
5 Enzymatic Selectivity in Synthetic Methods.
6 Aldolase Enzymes for Complex Synthesis.
7 Enzymatic Synthesis of Glycosides and Glucuronides.
8 Synthesis of Cyanohydrins Using Hydroxynitrile Lyases.
9 Synthesis of Chiral sec-Alcohols by Ketone Reduction.
10 Reduction of Functional Groups.
11 Enzymatic Oxidation Chemistry.
Schrader).
Zambianchi).
12 Whole-cell Oxidations and Dehalogenations.
Index.
