This is your "Laboratory Guide" for successful electrophoretic separations ! Organized in two parts it gives the reader a thorough presentation of the fundamentals followed by a detailed description of 15 of the most common methods currently in use. The Third Edition now includes the latest developments in 2D-electrophoresis as well as an overview of the proteome analysis methodology. From reviews of the previous editions: "...The rigorous description of each method along with the extensive figures will easily allow the novice to reproduce these methods in the laboratory..." --The Analyst "...Perhaps the most important point about the book is that all the recipes provided do actually work...--Journal of Laboratory Medicine "...an excellent book which we recommend greatly and which has not to be missed in any laboratory of cellular and molecular biology which respects it self!" --Cellular and Molecular Biology "...As a comprehensive guide to the huge variety of electrophoretic methods now available, this is very good value...The superb...troubleshooting appendix almost justifies the price on ist own..." --Laboratory Equipment Digest
Rezensionen / Stimmen
"...an excellent reference to help the reader to become acquainted with the field of electrophoresis." (Clinical Chemistry) "...a practical guide..." (SciTech Book News, Vol. 26, No. 2, June 2002)
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ISBN-13
978-3-527-30300-7 (9783527303007)
Schweitzer Klassifikation
Part I Fundamentals: electrophoresis; isotachophoresis; isoelectric focusing; blotting; interpretation of electropherograms; proteome analysis; instrumentation; equipment for part II. Part II Methods: method 1 - PAGE of dyes; method 2 - agarose and immuno electrophoresis; method 3 - titration curve analysis; method 4 - native PAGE in amphoteric buffers; method 5 - agarose IEF; method 6 - PAGIEF in rehydrated gels; method 7 - horizontal SDS-PAGE; method 8 - vertical PAGE; method 9 - semi-dry blotting of proteins; method 10 - IEF in immobilized pH gradients; method 11 - high-resolution 2D electrophoresis; method 12 - PAGE of double stranded DANN; method 13 - native PAGE of single stranded DNA7; method 14 - denaturing gradient gel electrophoresis; method 15 - denaturing PAGE of DANN. Appendix: Trouble-shooting guide.