Part I Classical Cytogenetics: 01 Tissue Culture, Friedel Wenzel; 02 Chromosome staining, Angelika Köhler; 03 Karyotyping and Data Interpretation, Karsten Held; 04 Documentation, Peter Miny, Rolf-Dieter Wegner; Part II Postnatal Diagnosis: 05 Peripheral Blood, Iris Bartels; 06 Establishment of permanent growing lymphoblastoid cell lines, Heidemarie Neitzel; 07 Solid tissues, Regine Schubert and Gesa Schwanitz; 08 Cells from urine sample, Hannelore Körner, Henrike Dia, Christiane Bommer; 09 Classical and molecular cytogenetics of tumor cells, Brigitte Schlegelberger, Simone Metzke, Svetlana Harder, Reina Zühlke-Jenisch,Yanming Zhang, Reiner Siebert; 10 Cytogenetics of Meiotic Cells, R. Johannisson; Part III Prenatal Diagnosis: 11 Prenatal Diagnosis - an introduction, Rolf-Dieter Wegner; 12 Amniotic fluid cell analysis, Ingo Kennerknecht, Mahmoud Djalali, Gotthold Barbi, Walter Just, Walther Vogel; 13 Chorionic villi sampling, Rolf-Dieter Wegner and Holger Toennies; Part IV Special Applications: 14 Diagnosis of chromosomal instability syndromes, Rolf-Dieter Wegner, Markus Stumm; 15 Flow Cytometric Testing for Syndromes with Chromosomal Instability and for Aplastic Anemia and Related Hematological Disorders, Detlev Schindler and Holger Hoehn; 16 Cell fusion, Markus Stumm and Rolf-Dieter Wegner; 17 Origin of Trisomies, Gesa Schwanitz and Thomas Eggermann; Part V Molecular Cytogenetics: 18 Fluorescence in situ hybridization, Gesa Schwanitz and Regine Schubert; 19 Fluorescence in situ Hybridization (FISH) Analysis in Human Sperm Cells, Evelyn Ko and Rene Martin; 20 Microdissection and Reverse Chromosome Painting, G. Senger, I. Chudoba, J. Weimer and U. Claussen; 21 Comparative genomic hybridization (CGH), Traudl Henn, Oskar A. Haas; Part VI Techniques in Development: 22 Fetal cells in maternal blood, Dorothee Gänshirt, Henk S.P.Garritsen, Wolfgang Holzgreve; 23 Spectral Karyotyping in Clinical and Tumor Cytogenetics, Evelin Schröck, Yuval Garini, Michael Köhler andThomas Ried; 24 Chromosome analysis by multiplex-FISH (M-FISH), Michael R. Speicher