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This volume explores the latest advancements made in the field of mass spectrometry (MS) based single cell proteomics. The chapters in this book focus on technologies, methods, and biological applications and together cover the entire single cell proteomics analysis workflow, from sample collection and preparation through liquid chromatographic separation and mass spectrometric data acquisition to data analysis and interpretation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Mass Spectrometry Based Single Cell Proteomics: Methods and Protocols is a valuable resource for all researchers interested in using these technologies and methods in their laboratories to continue making biological discoveries in this important field.
Label-Free Sample Preparation for Single Cell Proteomics.- Infrared Laser Ablation and Capture of Biological Tissue.- Comprehensive Micro-SPE-Based Bottom-Up Proteomic Workflow for Sensitive Analysis of Limited Samples.- A Sample Preparation Procedure for Isobaric Labelling-Based Single Cell Proteomics.- Nanoliter-Scale Sample Preparation for Single Cell Proteomic Analysis Using Glass-Oil-Air-Droplet Chip.- Fully Integrated Online Strategy for Highly Sensitive Proteome Profiling.- Efficient and Sensitive Sample Preparation, Separations, and Data Acquisition for Label-Free Single-Cell Proteomics.- Robust Surfactant-Assisted One-Pot Sample Preparation for Label-Free Single-Cell and Nanoscale Proteomics.- Spatial Proteomics of Single Hepatocytes with Multiplexed Data-Independent Acquisition (mDIA).- Profiling Mouse Brain Single-Cell-Type Proteomes via Adeno-Associated Virus-Mediated Proximity Labeling and Mass Spectrometry.- EquiCP: Targeted Single Cell Proteomics by Mass Spectrometry with Isobaric Labeled Multiplexing.- Analyzing Post-Translational Modifications in Single Cells.- Single Cell Proteomics Analysis with Tecan Uno and SCREEN Workflow.- Standardized Workflow for Mass Spectrometry-Based Single-Cell Proteomics Data Processing and Analysis Using the scp Package.- Bioinformatics Pipeline for Processing Single Cell Data.
