Preface Acknowledgements. List of Contributors. Part I Techniques for studying gene expression. Chapter 1. Gene expression, basic principles (I. Gozes). Chapter 2. An approach to in situ hybridization using oligonucleotide cDNA probes (G.R. Uhl). Chapter 3. Non-radioactive in situ hybridization (A.H.N. Hopman, A.K. Raap, J.E. Landegent, J. Wiegant, R.H. Boerman and M. Van der Ploeg). Chapter 4. Expression cloning of cDNAs encoding neuropeptide precursors (M.J. Low, S. Cooperman, G. Mandel and R.H. Goodman). Chapter 5. The relevance of DNA mediated gene transfer in mammalian cells for neuroscience (M.J. Low, J.S. Fink, T. Tsukada, M. Verhave, G. Mandel and R.H. Goodman). Part II Techniques for studying receptor localization. Chapter 6. A laboratory guide for the in vitro labeling of receptors in tissue sections for autoradiography (J.M. Palacios, R. Cortes and M.M. Dietl). Chapter 7. Influence of tissue treatment on quantitative receptor autoradiography (M. Herkenham). Chapter 8. Quantitative autoradiography in neuroscience (A.P. Davenport, I.J.M. Beresford, M.D. Hall, R.G. Hill and J. Hughes). Chapter 9. The use of automated image analysis for quantitative receptor autoradiography (A. Schleicher and K. Zilles). Chapter 10. Strategies for raising antibodies against benzodiazepine receptors (G. Richards, P. Schoch, P. Haring, B. Takacs and H. Mohler). Chapter 11. Anti-receptor anti-iotypic antibodies (P.O. Couraud and A.D. Strosberg). Chapter 12. Ligand-anti-ligand immunocytochemistry for peptidergic binding site localization in rat kidney and brain (R. Ravid and G.J. Boer). Chapter 13. Mismatches between receptor and transmitter localizations in the brain (M. Herkenham). Part III Techniques for studying antigen localization. Chapter 14. Antigen identity in immunocytochemistry (C.W. Pool and R.M. Buijs). Chapter 15. Colloidal gold probes in light microscopy: present state and prospects for the future (J. De Mey). Chapter 16. Press-blotting on gelatin-coated nitrocellulose membranes after gel isoelectric focusing: applications in the detection of peptides in the brain and the characterization of antiserum specificity (P.J. Van der Sluis, C.W. Pool and A.A. Sluiter). Chapter 17. Conjugation of antibodies and enzymes (D.M. Boorsma). Chapter 18. Immunoenzyme double staining (D.M. Boorsma and H.W.M. Steinbusch). Chapter 19. An introduction to immunoelectron microscopy in neuroscience (F.W. Van Leeuwen). Chapter 20. Silver intensification of colloidal gold or horseradish peroxidase for dual ultrastructural immunocytochemistry (A.N. Van den Pol). Part IV Combinations and applications of molecular neuroanatomical techniques. Chapter 21. Immunocytochemical demonstration of GABA in physiologically characterized, HRP-filled neurons and their postsynaptic targets (P. Somogyi). Chapter 22. The Phaseolus vulgaris-leucoagglutinin (PHA-L) anterograde axonal tract method (C.R. Gerfen and P.E. Sawchenko). Chapter 23.
