Today, fluorescence spectroscopy is an important tool of investigation in many areas. In analytical sciences, its advantage is extremely high sensitivity and selectivity - even single molecules can be detected - and it achieves a high spatial resolution and time resolution in combination with microscopic techniques or laser techniques, respectively. In material sciences, this is used to study structure and dynamics of surfaces. Particularly in the areas of biochemistry and molecular genetics, fluorescence spectroscopy has become a dominating technique. Together with the latest imaging techniques, fluorescence spectroscopy allows a real-time observation of the dynamics of intact biological systems with an unprecedented resolution.
This book offers a comprehensive introduction to and survey of fluorescence spectroscopy. It is written for newcomers and active researchers alike who are learning to apply fluorescence methods in the areas of chemistry, physical chemistry, polymers, materials, colloids, biochemistry, biology, medical and pharmaceutical research.
Rezensionen / Stimmen
"...explains the principles and application of fluorescence spectroscopy for investigating the structure and dynamics of matter and living systems..." (SciTech Book News, Vol. 26, No. 2, June 2002)
Auflage
Sprache
Verlagsort
Zielgruppe
Für höhere Schule und Studium
Für Beruf und Forschung
Physikochemiker, Photochemiker, Molekülphysiker, Komplexchemiker, Dozenten der Chemie
Illustrationen
162
22 s/w Tabellen, 1 farbige Abbildung, 162 s/w Abbildungen
Illustrations (some col.)
Maße
Höhe: 24 cm
Breite: 17 cm
Dicke: 23 mm
Gewicht
ISBN-13
978-3-527-29919-5 (9783527299195)
Schweitzer Klassifikation
Introduction
Absorption of UV-visible light
Characteristics of Fluorescence Emission
Effects of Photophysical or Photochemical Inter- or Intramolecular Processes on Fluorescence Emission
Fluorescence Polarization. Emission Anisotropy
Principles of Fluorometric Techniques
Effect of Polarity on Fluorescence Emission. Probes of Polarity
Fluidity, Order Parameters, Molecular Mobility. Estimation by Means of Fluorescent Probes
Resonance Energy Transfer and Applications
Fluorescent Molecular Sensors of Ions and Molecules
Advanced Fluorometric Techniques