Although the biotechnological application of bacterial cells has vastly increased in recent years, the use of mammalian cells is still very limited. This book supplies the fundamentals for biotechnological work with mammalian cells. Topics covered include: the raising of useful cell lines, the exploitation of mammalian cell vectors, the production of biological products and safety measures needing to be observed. This book, written by renowned experts, addresses scientists and graduate students working on biotechnological problems, as well as government officials who are concerned with biotechnology.
Sprache
Verlagsort
Verlagsgruppe
Zielgruppe
Für höhere Schule und Studium
Für Beruf und Forschung
Illustrationen
50
14 s/w Tabellen, 50 s/w Abbildungen
50 black & white illustrations, 14 black & white tables, biography
Gewicht
ISBN-13
978-3-540-18006-7 (9783540180067)
Schweitzer Klassifikation
Contents:
Raising of Useful Cell Lines: Cultivation of Human Myeloma Cells. Characteristics and Utility of a Panel of 93 Human Leukemia-Lymphoma Cell Lines. Structure and Function of E1A Genes of Adenovirus Type 12 - Application of E1A for Raising a Useful Mammalian Cell Line. A Role for Epstein-Barr Virus (EBV) in Immortalization of Primate Epithelial Cells.- Exploration of Mammalian Cell Vectors: L Factor, a Potentially Useful Plasmid Vector for Mammalian Cells. Development and Application of a Vector System that Permits Cloning of cDNA's on the Basis of their Expression in Mammalian Cells.- Production of Biologicals and the Safety Problems: Production and Development of Biologicals Derived from Recombinant-DNA Mammalian Cell Cultures. Production of Monoclonal Antibodies with Human-Human Hybridomas in Serum-Free Culture and its Prospects for Medical Use. Mass Mammalian Cell Culture. Production and Characterization of Human Interferon- Gamma and Other Substances Produced by Genetically Engineered Mammalian Cells. The Safety of Products Derived from Continuous Cell Lines.- Author Index.- Subject Index.
