Part I. Keratinocyte and Organ Cultures
Primary Mouse Keratinocyte Culture
Annalisa Pirrone, Barbara Hager, and Philip Fleckman
Serial Cultivation of Primary Adult Murine Keratinocytes
Richard P. Redvers and Pritinder Kaur
Keratinocyte Culture in the Absence of Substrate Attachment
Monika Jost and Ulrich Rodeck
Application of Genetically Modified Feeder Cells for Culture of Keratinocytes
Takashi Kameda and Toshihiro Sugiyama
Organ Culture of Developing Mouse Skin and Its Application for Molecular Mechanistic Studies of Morphogenesis
Mariko Kashiwagi and Nam-ho Huh
Experimental Models to Analyze Differentiation Functions of Cultured Keratinocytes In Vitro and In Vivo
Nicole Maas-Szabowski, Norbert E. Fusenig, and Hans-Jürgen Stark
In Vitro Fabrication of Engineered Human Skin
Alexander Margulis, Weitian Zhang, and Jonathan A. Garlick
Part II. Epidermal Stem Cells
In Vivo Labeling and Analysis of Epidermal Stem Cells
Wei-Yang Wu and Rebecca J. Morris
Method for the Harvest and Assay of In Vitro Clonogenic Keratinocytes Stem Cells From Mice
Wei-Yang Wu and Rebecca J. Morris
FACS Enrichment of Human Keratinocyte Stem Cells
Amy Li and Pritinder Kaur
Isolation, Characterization, and Culture of Epithelial Stem Cells
Jackie R. Bickenbach
Keratin 19 as a Stem Cell Marker In Vivo and In Vitro
Danielle Larouche, Cindy Hayward, Kristine Cuffley, and Lucie Germain
Part III. Analysis of Epidermal Differentiation
Immunolocalization in the Epidermis
Tammy-Claire Troy, Ramtin Rahbar, Bilge Diker, and Kursad Turksen
Epidermal Cell Analysis by RT-PCR
Tammy-Claire Troy, Robert Man-Kit Cheung, and Kursad Turksen
Whole-Mount Assays for Gene Induction and Barrier Formation in the Developing Epidermis
Carolyn Byrne and Matthew J.Hardman
Analysis of Early Epidermal Development in Zebrafish
Ashley E. Webb and David Kimelman
Analysis of E2F Factors During Epidermal Differentiation
Wing Y. Chang and Lina Dagnino
Analysis of HOX Homeodomain Proteins and Gene Transcripts in the Epidermis
László G. Kömüves and Corey Largman
Apoptosis in the Epidermis
Kiyofumi Yamanishi, Chun-Shen Shen, and Hitoshi Mizutani
Fate of Desmosomal Proteins in Apoptotic Epidermal Cells
Jörg Weiske and Otmar Huber
Analysis of Connexin 43 Expression on Keratinocytes Using Flow Cytometry
Maja Matic, Christopher Pullis, Marc G. Golightly and Sanford R. Simon
MMP-9 and TIMP-1 Assays in Keratinocyte Cultures
Takashi Kobayashi
Characterization of Epithelial Cells in the Hair Follicle With S100 Proteins
Kenji Kizawa and Mayumi Ito
Immunoelectron Microscopic Analysis of Cornified Cell Envelopes and Antigen Retrieval
Akemi Ishida-Yamamoto
Part IV. Methods and Approaches for the Analysis for Epidermal Function
Cell Kinetic Analysis in Artificial Skin Using Immunochemical Methods
Andrea Casasco, Antonia Icaro Cornaglia, Federica Riva, Marco Casasco, and Alberto Calligaro
Proliferation, Differentiation, and Inflammation in Normal and Hyperproliferative Skin Using Multiparameter Flow Cytometry
Piet E. J. van Erp
Fluorimetric DNA Assay of Cell Number
William R. Otto
Keratinocyte Transient Transfections
Anthony M. Flores and Brian J. Aneskievich
Tetracycline-Regulated Gene Expression in Epidermal Keratinocytes
Richard B. Presland and Philip Fleckman
Gene Targeting by Oligonucleotides in Keratinocytes
Olga Igoucheva and Kyonggeun Yoon
Promoter Analysis in the Human SPRR Gene Family
David F. Fischer and Claude Backendorf
Stable Integration of Large PAC
