Neuronal Morphogenesis

Culturing primary cortical neurons for live-imaging.- Electron Microscopy of Neurons on Biomimetic Substrates.- Analysis of Neurite and Spine Formation in Neurons In Vitro.- Quantifying Dendritic Arbors In Vitro and In Vivo in Rodent Models.- Visualizing and Measuring Dendrite Arborization in Drosophila Somatosensory Neurons.- Analyses of Neurite and Spine Formation by In Utero Electroporation in Mice.- Analysis of Neuronal Morphology by Two-Photon Microscopy.- In-Ovo Electroporation of Embryonic Chicken Spinal Cord in combination with Ex-Vivo Slice Culture for Live Imaging of Vertebrate Neuronal Differentiation.- In Vitro and In Vivo Analysis of Neuronal Polarity.- Proteomic Identification and Label-Free Quantification of Proteins Implicated in Neurite and Spine Formation.- 3D reconstruction of the mitochondrial network within the neuronal soma from SBF-SEM volume data.- DNeuroMAT: A Deep-Learning Based Neuron Morphology Analysis Toolbox.- Ex Vivo Live Imaging in Brain Slices for Analysis of Neurite Formation in Combination with In Utero Electroporation.- Imaging of Structural Plasticity of Dendritic Spines with Two-Photon Microscopy.- Visualization and Quantification of Organelle Axonal Transport in Cultured Neurons.- Visualization of microtubule polymerization during axon outgrowth using fluorescently labelled microtubule plus-end tracking proteins.- Interrogating the molecular clutch in neuronal growth cones: measuring traction forces, F-actin retrograde flow and point contact demographics.- Measuring Retrograde Actin Flow in Neuronal Growth Cones.- Morphological Analysis of Neurons and Glia using Mosaic Analysis with Double Markers.- Isolation and Culture of Adult Rat Dorsal Root Ganglion Neurons for the In Vitro Analysis of Peripheral Nerve Degeneration and Regeneration.- A live imaging assay for regenerating peripheral neuron.- Nerve Regeneration Using Compartmentalized system in CIPN model.- Live imaging analysis of axonal regeneration in human iPSC-derived motor neurons using a microfluidic system.- In Vivo Neural Tract Tracing Procedures: Unravelling Neural Hodology Using Fluorescent and non-fluorescent neural tract tracers, Optogenetic Approach, and Diffusion Tensor Neurotractography Protocols.