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This volume provides comprehensive knowledge on the fundamental techniques and detailed laboratory protocols used in stem cell research and their potential application in the field of neuroscience. The chapters in this book cover detailed descriptions of techniques for isolation, maintenance, differentiation, and characterization of neural stem cells, and the development of in vitro stem cell-based neurological disease model. There are detailed methods on leading techniques for stem cell genetic engineering and intra-organ transplantation for research and therapeutic purposes, in vivo imaging of donor stem cells, and essential requirements for setting up a new stem cell laboratory. Neuromethods series offers chapters with key advice and procedure specifics to empower the readers to successfully achieve their own scientific and experimental goals. Cutting-edge and pragmatic, Stem Cell Technologies in Neuroscience is a valuable resource for academic and translational researchers, industry scientists, biology teachers, and life science graduate students to grasp the vital pulse of the rapidly growing field of stem cell research.
Three-Dimensional Cultures of Human Neural Stem Cells: An Application for Modeling Alzheimer's Disease Pathogenesis.- Neural Stem Cell Fate Control on Micropatterned Substrates.- Dopaminergic and GABAergic Neuron In Vitro Differentiation from Embryonic Stem Cells.- Transfection of Cultured Primary Neurons.- Reprogramming of Mouse Fibroblasts to Induced Oligodendrocyte Progenitor Cells.- Isolation and Culture of Oligodendrocyte Precursor Cells from Pre- and Postnatal Rodent Brain.- Schwann Cell Isolation and Culture Reveals the Plasticity of these Glia.- Generation of Cerebral Organoids Derived from Human Pluripotent Stem Cells.- Development of Mouse Cell Based In Vitro Blood-Brain Barrier Models.- Microfluidic Device for Studying Traumatic Brain Injury.- Multimodal Neural Stem Cell Research Protocols for Experimental Spinal Cord Injuries.- Real-Time Dual MRI for Predicting and Subsequent Validation of Intra-Arterial Stem Cell Delivery to the Central Nervous System.- Standardized Cryopreservation of Stem Cells.- Isolation and Characterization of Extracellular Vesicles in Stem Cell-Related Studies.- Essential Requirements for Setting Up a Stem Cell Laboratory.
