The ERK Cascade As a Prototype of MAPK Signaling Pathways
Hadara Rubinfeld and Rony Seger
Determination of ERK Activity: Antiphospho-ERK Antibodies, In Vitro Phosphorylation, and In-Gel Kinase Assay
Sarah Kraus and Rony Seger
Detection of ERK1/2 Activities Using Affinity Reagents
Rafael Pulido, Ángel Zúñiga, and Axel Ullrich
Activation of SAPKs/JNKs and p38s In Vitro
John M. Kyriakis, Hong Liu, and Deborah N. Chadee
Investigating the Cellular BMK1/ERK5 Signaling Pathway
Richard I. Tapping, Kato Yutaka, Ta-Hsiang Chao, Masaaki Hayashi, Jeng-Fan Lo, Sung-Woo Kim, and Jiing-Dwan Lee
Pull-Down Assays for Guanosine 5'-Triphosphate-Bound Ras-Like Guanosine 5'-Triphosphatases
Miranda van Triest and Johannes L. Bos
Regulation of MAPK Cascades by Protein Tyrosine Phosphatases
Josema Torres, Carmen Blanco-Aparicio, and Rafael Pulido
Use of Inhibitors in the Study of MAPK Signaling
Yoav D. Shaul and Rony Seger
Structure of MAPKs
Elizabeth J. Goldsmith, Melanie H. Cobb, and Chung-I Chang
Subcellular Localization of MAPKs
Makoto Adachi and Eisuke Nishida
Study of MAPK Signaling Using Knockout Mice
Gilles Pagès and Jacques Pouysségur
Computer Simulation of MAPK Signal Transduction
Baltazar D. Aguda and Herbert M. Sauro
Signaling by Growth Factor Receptors
Bose S. Kochupurakkal and Yosef Yarden
Activation of MAPK by G Protein-Coupled Receptors
Piero Crespo and J. Silvio Gutkind
Identification of MAPK Substrates by Expression Screening With Solid-Phase Phosphorylation
Rikiro Fukunaga and Tony Hunter
Study of Substrate Specificity of MAPKs Using Oriented Peptide Libraries
Michael B. Yaffe
Ras Signaling Pathway for Analysis of Protein-Protein Interactions in Yeast and Mammalian Cells
Ami Aronheim
Methods inFunctional Proteomics: Two-Dimensional Polyacrylamide Gel Electrophoresis With Immobilized pH Gradients, In-Gel Digestion, and Identification of Proteins by Mass Spectrometry
Karine R. Bernard, Karen R. Jonscher, Katheryn A. Resing, and Natalie G. Ahn
Practical Methods for Deuterium Exchange/Mass Spectrometry
Andrew N. Hoofnagle, Katheryn A. Resing, and Natalie G. Ahn
Use of Xenopus Oocytes and Early Embryos to Study MAPK Signaling
Eusebio Perdiguero and Angel R. Nebreda
MAPK Cascades in the Brain: Lessons From Learning
Diego E. Berman and Yadin Dudai
Index
