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This volume focuses on protein analysis covering a wide spectrum of the utility of mass spectrometry within neurobiological disciplines. The chapters in this book discuss label (iTRAQ, TMT, protein arrays) and label-free workflows (SWATH, MALDI Imaging, and label-free quantitation). Other chapters look at experimental strategies targeted to the identification and quantitation of specific lipids and post-translational modifications, as well as proteomic workflows that focus on characterization of subcellular proteomes. The last few chapters in the book describe various bioinformatics pipelines used to analyze the molecular data derived from high-throughput transcriptomic and proteomic experiments on brain tissue. The Neuromethods series offers chapters with key advice and procedure specifics to empower the readers to successfully achieve their own scientific and experimental goals. Thorough and comprehensive, Current Proteomic Approaches Applied to Brain Function is a valuable resource for graduate students and postdoctoral fellows interested in neuroproteomics, as well as researchers looking for further insight into the growing field of mass spectrometry in neuroscience.
Brain Proteomics: Decoding Neuroproteomes using Mass-Spectrometry.- Applications of Amine-Reactive Tandem Mass Tags (TMT) in Human Neuroproteomics.- Application of Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) to Monitor Olfactory Proteomes during Alzheimer´s Disease Progression.- Protein Microarrays in Neurodegenerative Diseases.- Comprehensive Shotgun Proteomic Analyses of Oligodendrocytes using Ion Mobility and Data Independent Acquisition.- Non-Targeted Brain Lipidomics Profiling Performed by UPLC-ESI-qTOF-MS/MS.- Methods for Human Olfactory Bulb-Tissue Studies using Peptide/Protein MALDI-TOF Imaging Mass Spectrometry (MALDI-IMS).- Neuroproteomics using Short GeLC-SWATH: From the Evaluation of Proteome Changes to the Clarification of Protein Function.- Analysis of Brain Phosphoproteome using Titanium Dioxide Enrichment and High Resolution LC-MS/MS.- N-Glycomics and N-Glycoproteomics of Human Cerebrospinal Fluid.- InVivo Strategies to Isolate and Characterize the Neuronal Ubiquitinated Proteome.- Characterization of the Phosphoproteome and Sialoproteome in Brain Tissues by Mass Spectrometry.- Proteomic Analysis of SUMOylation in the Post-Ischemic Brain.- S-Nitrosylation in Alzheimer´s Disease using Oxidized Cysteine-Selective cPILOT.- Proteomic Analysis of Extracellular Vesicles in Neurological Diseases.- Quantitative In-Depth Profiling of the Postsynaptic Density Proteome to Understand the Molecular Mechanisms Governing Synaptic Physiology and Pathology.- Nuclear Proteomics for Exploring MK-801 Treated Oligodendrocytes to Better Understand Schizophrenia.- Localized Proteomics of Individual Neurons Isolated from Formalin-Fixed, Paraffin Embedded Tissue Sections using Laser Capture Microdissection.- Creation of Reusable Bioinformatics Workflows for Reproducible Analysis of LC-MS Proteomics Data.- Integration of Transcriptomic and Proteomic Data for Disease Insights.
