This book is the updated English version of the 2006 German bestseller 'Zelluläre Diagnostik', a comprehensive presentation of flow cytometry and its applications. While some techniques of immunophenotyping by flow cytometry already are routine procedures in the laboratory, new methods for the functional characterization of cells, the analysis of rare cells, and the diagnosis of complex materials have only begun to win wide recognition. New approaches such as slide-based cytometry will lead to an increase in the use of cytometric techniques. Multiparameter approaches will further improve analysis. The book provides a comprehensive and detailed compilation of all aspects of flow cytometry in research and the clinic. For newcomers it offers a thorough introduction, for advanced users, specific protocols and interpretation assistance.
Sprache
Verlagsort
Verlagsgruppe
Zielgruppe
Illustrationen
184 fig., 29 in color, 117 tab.
Maße
Höhe: 26 cm
Breite: 20.5 cm
Gewicht
ISBN-13
978-3-8055-8555-2 (9783805585552)
Schweitzer Klassifikation
From the Contents; Background and Methodological Principles; History; principles; selection and combination of fluorescent dyes; FRET; intracellular antigens; cell sorting; data analysis; quality control; Characterization and Phenotyping of Cells; T and B lymphocytes; NK cells; age-adjusted normal values; dendritic cells; stem cells; rare events such as mesenchymal progenitors; Cell Function Analysis; Platelet function; calcium and pH; phagocytosis and oxidative burst; multidrug resistance; DNA and proliferation analyses; antigen-specific T cells; Diagnostic Indications; Congenital and acquired immunodeficiencies; transplanted patients; sepsis; paroxysmal nocturnal hemoglobinuria; bronchoalveolar lavage fluid; leukemias and lymphomas; cross-match analysis; blood products.
