Part I. Reviews
Specificity and Performance of Diagnostic PCR Assays
Konrad Sachse
Pre-PCR Processing of Samples
Peter Rådström, Rickard Knutsson, Petra Wolffs, Maria Dahlenborg, and Charlotta Löfström
Critical Aspects of Standardization of PCR
Jeffrey Hoorfar and Nigel Cook
PCR Technology and Applications to Zoonotic Food-Borne Bacterial Pathogens
Peter Stephensen Lübeck and Jeffrey Hoorfar
Part II. Protocols
Detection, Identification, and Subtyping of Actinobacillus pleuropneumoniae
Joachim Frey
Identification and Differentiation of Brucella abortus Field and Vaccine Strains by BaSS-PCR
Darla R. Ewalt and Betsy J. Bricker
Isolation of Campylobacter and Identification by PCR
Mark D. Englen, Scott R. Ladely, and Paula J. Fedorka-Cray
Detection and Differentiation of Chlamydiae by Nested PCR
Konrad Sachse and Helmut Hotzel
Detection of Toxigenic Clostridia
Michel R. Popoff
PCR-Based Detection of Coxiella burnetii from Clinical Samples
Mustapha Berri, Nathalie Arricau-Bouvery, and Annie Rodolakis
Detection and Subtyping of Shiga Toxin-Producing Escherichia coli (STEC)
Peter Gallien
Detection of Listeria monocytogenes Using a PCR/DNA Probe Assay
Louise O'Connor
Detection of Leptospira interrogans
John W. Lester and Rance B. LeFebvre
Detection of Pathogenic Mycobacteria of Veterinary Importance
Robin A. Skuce, M. Siobhan Hughes, Malcolm J. Taylor, and Sydney D. Neill
Multiplex PCR of Avian Pathogenic Mycoplasmas
Mazhar I. Khan
Detection and Differentiation of Ruminant Mycoplasmas
Helmut Hotzel, Joachim Frey, John Bashiruddin, and Konrad Sachse
Detection of Mycoplasma hyopneumoniae from Clinical Samples and Air
Marylène Kobisch and Joachim Frey
PCR-Detection of Hemophilusparagallinarum, Hemophilus somnus, Mannheimia (Pasteurella) hemolytica, Mannheimia spp., Pasteurella trehalosi, and Pasteurella multocida
Henrik Christensen, Magne Bisgaard, Jesper Larsen, and John Elmerdahl Olsen
Detection of Salmonella spp.
Burkhard Malorny and Reiner Helmuth
Detection of Toxoplasma gondii
Jonathan M. Wastling and Jens G. Mattsson
PCR-Derived Methods for the Identification of Trichinella Parasites from Animal and Human Samples
Edoardo Pozio and Giuseppe La Rosa
Detection of Pathogenic Yersinia enterocolitica by a Swab Enrichment PCR Procedure
Rickard Knutsson and Peter Rådström
Index
