List of Contributors xii
Preface xvi
1 Reactive Oxygen Species Signaling from the Perspective of the Stem Cell 1
Saghi Ghaffari and Raymond Liang
1.1 Introduction 1
1.2 ROS Regulation 2
1.3 ROS Signaling 3
1.4 ROS and Stem Cells 5
1.5 ROS, Metabolism, and Epigenetic Influence 9
1.6 Stem Cells and Mitochondria 9
1.7 ROS and Stem Cell Aging 12
2 Analysis of Protein Carbonylation 24
Ashraf G. Madian, Fred E. Regnier, and Ao Zeng
2.1 Introduction 24
2.2 In Vivo Carbonylation Reactions 27
2.3 Analytical Derivatization of Carbonylated Groups 34
2.4 Selective Purification and/or Detection of Carbonylated Proteins and Peptides 36
2.5 Oxidative Stress?]Based PTMS Not Involving Carbonylation 38
3 Diversity of Protein Carbonylation Pathways: Direct Oxidation,Glycoxidation, and Modifications by Lipid Peroxidation Products 48
Maria Fedorova
3.1 Introduction 48
3.2 Pathways of Protein Carbonylation 49
3.3 Analytical Methods for Detection of Total and Specific Protein Carbonylation 57
3.4 Protein Susceptibility to Different Carbonylation Pathways and Modifications Cross?]Talk 67
4 Protein Carbonylation by Reactive Lipids 83
Koji Uchida
4.1 Introduction 83
4.2 Chemistry of Protein Carbonylation by Reactive Lipid Aldehydes 84
4.3 Antigenicity of Protein Carbonyls 87
4.4 Thiolation of Protein Carbonyls 89
4.5 Reductive Amination?]Based Fluorescent Labeling of Protein Carbonyls 91
5 Mechanism and Functions of Protein Decarbonylation 97
Yuichiro J. Suzuki
5.1 Protein Carbonylation 97
5.2 Primary Protein Carbonylation in Cell Signaling 98
5.3 Discovery and Mechanisms of Protein Decarbonylation 101
5.4 Proposed Functions of Protein Decarbonylation in Oxidative Stress and Redox Signaling 103
6 Carbonylated Proteins and Their Metabolic Regulation: Overview of Mechanisms, Target Proteins, and Characterization Using Proteomic Methods 110
Somaieh Afiuni?]Zadeh and Timothy J. Griffin
6.1 Metabolic Regulation and Reactive Oxygen Species 110
6.2 ROS and Protein Carbonylation 111
6.3 Metabolic Control and Characteristics of Carbonylated Proteins 113
6.4 Protein Targets of Carbonylation and Implications in Human Health 114
6.5 Technologies and Methods for Characterizing Protein Carbonylation 118
6.6 Emerging Multifunctional Reagents for Protein Carbonylation Analysis via MS 119
6.7 Emerging Methods for Characterizing Carbonylated Protein Networks and Affected Pathways 123
7 Oxidative Stress and Protein Carbonylation in Malaria 131
Maria Linares, Antonio Puyet, Amalia Diez, and Jose M. Bautista
7.1 Introduction 131
7.2 Oxidative Stress during Malaria Infection 132
7.3 Protein Carbonylation in Plasmodium and Oxidative Targeting of Antimalarials 137
7.4 Oxidative Dysfunction in Host Tissues 143
7.5 Host Tolerance to Malaria by Modulation of Oxidative Stress Responses 148
7.6 Perspectives 153
8 Protein Carbonylation in Brains of Subjects with Selected Neurodegenerative Disorders 167
Tanea T. Reed and D. Allan Butterfield
8.1 Introduction to Protein Carbonylation 167
8.2 Relationship between ROS and Oxidative Stress 169
8.3 An Overview of Some Neurodegenerative Diseases 171
8.4 Role of Protein Carbonylation in Brains of Subjects with AD 174
8.5 An Introduction to Tauopathies 185
8.6 An Introduction to Amyotrophic Lateral Sclerosis 186
9 Cigarette Smoke?]Induced Protein Carbonylation: Focus on Recent Human Studies 206
Graziano Colombo, Maria Lisa Garavaglia, Aldo Milzani, and Isabella Dalle?]Donne
9.1 Introduction 206
9.2 Protein Carbonylation in Human Smokers 212
9.3 Protein Carbonylation in Cultured Human Cell Models of Exposure to CS 218
9.4 Limitations and Congruence of In Vivo and In Vitro Human Studies 228
10 Chronic Obstructive Pulmonary Disease and Oxidative Damage 241
Esther Barreiro
10.1 Introduction 242
10.2 Protein Oxidation in Tissues 244
10.3 Antioxidants in Skeletal Muscle Fibers 247
10.4 Implications of Protein Carbonylation in COPD Skeletal Muscle Dysfunction 249
10.5 Muscle Protein Carbonylation and Exercise in COPD Patients 252
10.6 Protein Carbonylation in Muscles Exposed to Chronic Cigarette Smoke 253
10.7 Protein Carbonylation in Cancer Cachexia Models 255
10.8 Protein Carbonylation as a Predisposing Mechanism of Lung Cancer in COPD 257
11 Protein Carbonylation in Aging and Senescence 272
Jeannette Konig, Tobias Jung and Tilman Grune
11.1 Introduction 272
11.2 Protein Oxidation during Aging 274
11.3 Chemistry of Protein Carbonylation and Fate of Carbonylated Proteins 277
11.4 Protein Carbonyls in Cellular Aging Models 279
11.5 Protein Carbonylation in Aging Organisms 280
12 Adipose Carbonylation and Mitochondrial Dysfunction 291
Amy K. Hauck, Dalay H. Olson, Joel S. Burrill, and David A. Bernlohr
12.1 Introduction 291
12.2 Reactive Oxygen Species (ROS) 292
12.3 Oxidative Stress and Obesity 298
12.4 Detection of Protein Carbonylation 303
12.5 Outcomes of Protein Carbonylation 306
13 Protein Carbonylation in Plants 321
Ian Max Moller, Jesper F. Havelund, and Adelina Rogowska?]Wrzesinska
13.1 Introduction 322
13.2 Turnover of Reactive Oxygen Species in Plants 323
13.3 Methods Used in Plants for Quantifying and Identifying Carbonylation Sites 325
13.4 Protein Carbonylation in Plants 326
13.5 Protein Carbonylation in Plant Mitochondria 328
13.6 Protein Carbonylation in Seeds 333
14 Specificity of Protein Carbonylation and Its Relevance in Aging 340
Elisa Cabiscol, Jordi Tamarit, and Joaquim Ros
14.1 Introduction 340
14.2 Specificity of Protein Oxidative Damage 341
14.3 Protein Carbonylation in Aging 348
Index 384
