This manual is a comprehensive compilation of "methods that work" for deriving, characterizing, and differentiating hPSCs, written by the researchers who developed and tested the methods and use them every day in their laboratories. The manual is much more than a collection of recipes; it is intended to spark the interest of scientists in areas of stem cell biology that they may not have considered to be important to their work. The second edition of the Human Stem Cell Manual is an extraordinary laboratory guide for both experienced stem cell researchers and those just beginning to use stem cells in their work.
Auflage
Sprache
Verlagsort
Verlagsgruppe
Elsevier Science Publishing Co Inc
Zielgruppe
Für Beruf und Forschung
Scientists engaged in any aspect of stem cell research, as well as undergraduate and graduate students enrolled in laboratory courses.
Illustrationen
Maße
Höhe: 235 mm
Breite: 191 mm
ISBN-13
978-0-12-810101-8 (9780128101018)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Klassifikation
Suzanne Peterson received her doctoral degree from USC and immediately moved back to her home town of San Diego to pursue her Post-Doctoral work. A long-time member and the only native San Diegan of the Loring Laboratory at Scripps Research Institute, this summer Dr. Peterson followed her mentor Dr. Loring to Aspen Neuroscience and will serve as a senior scientist in the new founded lab. Her specialty is hematopoietic stem cells and she will continue to serve as co-editor of Human Stem Cell Manual: A Laboratory Guide with Dr. Jeanne Loring. Jeanne Loring is a world renown stem cell biologist, developmental neurobiologist, and geneticist. Dr. Loring's 3+ decades have spanned embryology and neurobiology, gene editing and stem cells, ultimately landing in regenerative medicine. Her decades of experience ranges from biotech, to pharma to bioscience and of course academia. She was the director for the Center for Regenerative Medicine and professor at Scripps Research Institute in La Jolla, CA for 12+ years. In June 2019 she founded Aspen Neuroscience, in La Jolla, where she and her staff will continue her work in regenerative medicine for those with currently untreatable neurological diseases. Their first target is a patient-specific neuron replacement therapy for Parkinson's disease. She will continue as Professor Emeritus and remains on the faculty of Scripps Research Institute. Dr. Loring has received numerous awards and accolades, including the 2015 Stem Cell Action Advocacy Award and 2015 Stem Cell Person of the Year.
Herausgeber*in
Senior Scientist, Scripps Research Institute, La Jolla, CA , USA
Director, Center for Regenerative Medicine and Professor, Scripps Research Institute, La Jolla, CA, USA
Part I: Basic methods: Maintenance of stem cell cultures
1.Human Embryonic Stem Cell Culture
2.Feeder layers and feeder-free culture
3.Mouse Embryonic Fibroblast Feeder Cells
4.Cryopreservation of hESC
Part II: Basic methods: Characterization of stem cells
5.Classical Cytogenetics: Karyotyping
6.Spectral Karyotyping and Fluorescent in situ Hybridization
7.Using SNP Genotyping to Identify Cell Lines, Determine Genomic Abnormalities and Monitor DNA Methylation Patterns
8.FACS/Cell sorting
9.Fluorescence Immunocytochemical Analysis of Stem Cells
10.Characterization of Stem Cells Using RT-PCR
11.Gene Expression Profiling of Stem Cells by Microarray
12.Teratomas produced from Human Embryonic Stem Cells Xenografted into Immunodeficient Mice
13.Generation of HESC-Derived Teratomas
Part III: Differentiation of stem cells
14."Embryoid Body? Formation and Neuroepithelia Differentiation
15.Motor Neuron and Dopamine Neuron Differentiation
16.Oligodendrocyte Differentiation from hESC
17.Cardiac
18.Hematopoietic
Part IV: Genetic manipulation of stem cells
19.Genetic Manipulation of hESC: Lentivirus Vectors
20.Methods developed for mouse ES cells
Part V: Advanced methods
21.Derivation of human ES cells
22.Neural Stem Cell Culture
23.Transplantation of stem cells
24.In vitro fertilization
25.Functional characterization-neural cells
Part VI: Practical issues in establishing a human ES cell laboratory
26.Setting Up a Facility for hESC Research
Part VII: Essays on stem cell biology
27.Intellectual Property: Owning the Stem Cell
28.Ethical Concerns for Stem Cell Research
29.Guidelines for hESC Research Oversight (ESCRO) Committees
