Molecular Diagnostics covers current molecular biological techniques used to identify the underlying molecular defects in inherited disease. Although an increasing number of laboratories, both academic and private are moving in that direction, there are only a few books in the existing literature, and they deal only partly with diagnosis at the molecular level. Each chapter includes the principle and a brief description of the technique, followed by exmples from the authors' own epertise. Contributors are well-known experts in their field, and derive from a variety of disciplines, to ensure breadth and depth of coverage.
Rezensionen / Stimmen
"...the book is uniform in style. The text is free of jargon and is well written in an easy to understand style. ...This book is a great resource for experienced readers, giving them a wide overview of the most up-to-date methods. It is also a great book for students and medical professionals who want to learn about the molecular tools used in diagnostics. ...a great teaching aid for courses involving molecular biology, genetics and diagnostic techniques." --AUSTRALIAN BIOCHEMIST (April 2006)
"...the book will be useful to investigators in the diagnostic laboratory and to scientists generally who want a summary of current methods. ...Inclusion of ethical and safety considerations (as well as a section on patents) is a distinct plus, not often seen. ...this is a well organized and edited contribution." --DOODY'S (October 2005)
Sprache
Verlagsort
Verlagsgruppe
Elsevier Science Publishing Co Inc
Zielgruppe
Für Beruf und Forschung
Postgraduate students, researchers, physicians and practicing scientists in molecular genetics; professionals from similar backgrounds working in diagnostic laboratories in academia or industry; academic institutions and hospital libraries
Produkt-Hinweis
Fadenheftung
Gewebe-Einband
Maße
Höhe: 290 mm
Breite: 223 mm
Dicke: 24 mm
Gewicht
ISBN-13
978-0-12-546661-5 (9780125466615)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Klassifikation
Dr. George Patrinos is an Associate Professor at the University of Patras School of Health Sciences (Department of Pharmacy) in Patras, Greece with Adjunct positions in Rotterdam, the Netherlands and Al-Ain, United Arab Emirates. His research interests span the fields of molecular diagnostics, high-throughput mutation screening, the development of online mutation diagnostic tools, and the implementation of genomics into healthcare, particularly for health systems in developing countries. George Patrinos has published more than 170 scientific papers in peer reviewed journals on topics related to genetics, genomic medicine, pharmacogenomics, molecular diagnostics, and social and economic evaluation for genomic medicine. Dr. Patrinos is also the co-author of Economic Evaluation in Genomic Medicine (2015) and co-Editor of Molecular Diagnostics, Second Edition (2009), both published by Elsevier, and serves as Communicating Editor for the journal Human Mutation. Additionally, he is co-organizer of the international meeting series "Golden Helix Symposia? and "Golden Helix Pharmacogenomics Days?. Prof. Dr. Wilhelm Ansorge is a Senior Research Scientist and coordinator of the Biochemical Instrumentation Programme at the European Molecular Biology Laboratory in Heidelberg, Germany. His research interests include the development of the first complete Human Genome microarray, with numerous applications in gene expression studies and high-throughput Molecular Diagnostics.
Herausgeber*in
Visiting Professor, Ecole Polytechnique Federale Lausanne, Switzerland
Chapter 1: Molecular Diagnostics: Past, Present, and Future
Section I: Molecular Diagnostic Technology
Chapter 2: Allele-Specific Mutation Detection by PCR-ARMS and PCR-ASO
Chapter 3: Competitive Oligopriming
Chapter 4: Oligonucleotide Ligation Assays for the Diagnosis of Inherited Diseases
Chapter 5: Enzymatic and Chemical Cleavage Methods to Identify Genetic Variation
Chapter 6: Mutation Detection by Single Strand Conformation Polymorphism and Heteroduplex Analysis
Chapter 7: Capillary Electrophoresis
Chapter 8: Temperature and Denaturing Gradient Gel Electrophoresis
Chapter 9: Two-Dimensional Gene Scanning
Chapter 10: Real-Time Polymerase Chain Reaction
Chapter 11: Pyrosequencing
Chapter 12: Molecular Cytogenetics in Molecular Diagnostics
Chapter 13: Detection of Genomic Duplications and Deletions
Chapter 14: Analysis of Human Splicing Defects Using Hybrid Minigenes
Chapter 15: DNA Microarrays and Genetic Testing
Chapter 16: Human Genome Microarray in Biomedical Applications
Chapter 17: Use of High Throughput Mass Spectrographic Methods to Identify Disease Processes
Chapter 18: The Application of Proteomics to Disease Diagnostics
Section II: Applications of Molecular Diagnostics and Related Issues
Chapter 19: Identification of Genetically Modified Organisms
Chapter 20: Pharmacogenetics and Pharmacogenomics: Impact on Drug Discovery and Development
Chapter 21: Molecular Diagnostic Applications in Forensic Science
Chapter 22: Molecular Diagnostics and Comparative Genomics in Clinical Microbiology
Chapter 23: Preimplantation Genetic Diagnosis
Chapter 24: Genetic Monitoring of Laboratory Animals
Chapter 25: The Use of Locus-Specific Databases in Molecular Diagnostics
Chapter 26: Safety Analysis in Retroviral Gene Therapy: Identifying Virus Integration Sites in Gene-Modified Cells
Chapter 27: Automated DNA Hybridization and Detection
Chapter 28: The Use of Microelectronic-Based Techniques in Molecular Diagnostic Assays
Chapter 29: Miniaturization Technologies for Molecular Diagnostics
Chapter 30: Human Gene Patents and Genetic Testing
Chapter 31: Genetic Counselling and Ethics in Molecular Diagnostics
Chapter 32: Genetic Testing and Psychology
Chapter 33: Safety in Biomedical and Other Laboratories
Chapter 34: Quality Management in the Laboratory
