This volume provides a comprehensive study of this key method of gel-based proteomics. Chapters in Difference Gel Electrophoresis: Methods and Protocols detail methods on principles of differential protein labeling, two-dimensional gel electrophoresis, techniques on optimized proteomic workflows, advanced mass spectrometry for protein identification, application of basic biological research, and applied biomarker discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Difference Gel Electrophoresis: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Reihe
Auflage
Softcover reprint of the original 1st ed. 2018
Sprache
Verlagsort
Zielgruppe
Illustrationen
24
20 s/w Abbildungen, 24 farbige Abbildungen
X, 318 p. 44 illus., 24 illus. in color.
Maße
Höhe: 254 mm
Breite: 178 mm
Dicke: 18 mm
Gewicht
ISBN-13
978-1-4939-8436-7 (9781493984367)
DOI
10.1007/978-1-4939-7268-5
Schweitzer Klassifikation