This first book to deal with this modern and powerful tool in instrumental analytical chemistry focuses on practical issues, making it a useful guide for both beginners and experienced scientists.
The first part describes the methodological background for the coupling of electromigrative separation techniques with mass spectrometry, discussing various separation techniques, interfacing options and MS detection systems. The second part focuses on method development, applications and aspects of quantification and validation. The whole is rounded off with a look at future developments.
Sprache
Verlagsort
Zielgruppe
Maße
Höhe: 240 mm
Breite: 170 mm
Dicke: 1700 mm
ISBN-13
978-3-527-32069-1 (9783527320691)
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Schweitzer Klassifikation
Christian Neusuess received his graduation at Heidelberg University and his Ph.D. at the University of Leipzig. He worked five years on applications of mass spectrometry with strong focus on capillary electrophoresis-mass spectrometry (CE-MS) at Bruker Deltonics in Bremen. Currently he is professor for general and analytical chemistry at Aalen University and engaged in CE-MS training in academia and industry. He is author of more than 30 publications and book chapters, mostly dealing with CE-MS. Carolin Huhn studied chemistry at the Philipps-University Marburg, Germany and at Massey University, Palmerston North, New Zealand. Currently, she is finishing her Ph.D. in analytical chemistry in Marburg. Her research interests focus on different capillary electromigrative separation techniques combined with sensitive and selective detection schemes for applications in the field of forensic and pharmaceutical science.
PART I: METHODOLOGY ELECTROMIGRATIVE SEPARATION MODES COUPLED TO MASS SPECTROMETRY Capillary Zone Electrophoresis (CE) Capillary Gel Electrophoresis (CGE) Capillary Isoelectric Focusing (CIEF) Capillary Electrokinetic Chromatography (CEC) Micellar Electrokinetic Chromatography (MEKC) Isotachophoresis (ITP) Multidimensional Separation Techniques INTERFACES Atmospheric Pressure Ionization (On-Line Coupling) Vacuum Ionization (Off-Line Coupling) MASS SPECTROMETER COUPLED TO ELECTROMIGRATIVE SEPARATION MODES Quadrupole Type Ion Traps Time-Of-Flight FTICR Others PART II: APPLICATIONS METHOD DEVELOPMENT General Considerations Influences of the Sample Composition Preconcentration Techniques Coatings SMALL CATIONIC SUBSTANCES Choice of Electrolyte and Interface Parameter Applications SMALL ANIONIC SUBSTANCES Choice of Electrolyte and Interface Parameter Applications BIOPOLYMERS Intact Proteins Oligonucleotides QUANTITATION AND VALIDATION Quantitation Rubustness and Precision Detection Limits Identification FUTURE DEVELOPMENTS Intrumentation Microchannel Electrophoresis - Mass Spectrometry ("Chip-MS") Applications
