PFGE is the most reliable method for separating large fragments of DNA and has had a major impact on biological research. This book presents detailed chapters on both the theory behind PFGE amd protocols for most of its major research and diagnostic applications. This book is intended for researchers at all levels in labs involved in molecular human genetics, mammalian genetics (mouse, rat, etc), molecular microbiologists, molecular parasitologists, biochemists who use molecular biology techniques, and some molecular diagnostic labs.
Rezensionen / Stimmen
an invaluable laboratory aid for all researchers using PFGE * International Journal of Biochemistry and Cell Biology, Vol. 28, No. 7, 1996 * All nine chapters treat all these problems which will certainly meet a great success. * Celular & Molecular Biology, Vol. 42, No. 3, 1996 *
Reihe
Sprache
Verlagsort
Zielgruppe
Illustrationen
halftones, line figures, tables
Maße
Höhe: 233 mm
Breite: 154 mm
Dicke: 11 mm
Gewicht
ISBN-13
978-0-19-963535-1 (9780199635351)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Klassifikation
Herausgeber*in
Senior Scientist, ICRF, Institute of Molecular MedicineJohn Radcliffe Hospital, Oxford
1. Theories of gel electrophoresis of high molecular weight DNA ; 2. PFGE in physical mapping ; 3. Mutation detection and diagnosis using PFGE ; 4. Cloning from PFGE-purified genomic DNA fragments and YACs ; 5. Yeast artificial chromosome cloning using PFGE ; 6. PFGE analysis of yeast artificial chromosomes ; 7. Functional analysis of mammalian genomes using yeast artificial chromosomes ; 8. PFGE in the study of a bacterial pathogen (Haemophilus influenzae) ; 9. Analysis of the genomes of protozoan parasites using PFGE
