This book explains in a clear consistent manner, some of the more commonly used protocols in neuroscience research. Each chapter is written by either the person who invented the procedure or an expert in the field. The format is uniform: "Overview," "Background," "Protocols," and "results and discussion." Each protocol begins with the principle of the technique, studies in cell culture, materials and reagents, and, lastly, step by step outline of the procedure itself. This highly practical book is also well illustrated (with 17 four color plates) to make the concepts and procedures easy to understand and perform.
Auflage
Sprache
Verlagsort
Zielgruppe
Für höhere Schule und Studium
Für Beruf und Forschung
Research
Produkt-Hinweis
Fadenheftung
Gewebe-Einband
Illustrationen
65
15 farbige Abbildungen, 65 s/w Abbildungen
XIV, 303 p. 80 illus., 15 illus. in color.
Maße
Höhe: 254 mm
Breite: 178 mm
Dicke: 19 mm
Gewicht
ISBN-13
978-0-387-95386-1 (9780387953861)
DOI
Schweitzer Klassifikation
Analyses of Intracellular Signal Transduction Pathways in CNS Progenitor Cells.- Confocal and Electron Microscopic Tracking of Internalized Neuropeptides and/or Their Receptors.- Transfection Methods for Neurons in Primary Culture.- Polyethylenimine: A Versatile Cationic Polymer for Plasmid-Based Gene Delivery in the CNS.- Transfection of GABAA Receptor with GFP-Tagged Subunits in Neurons and HEK 293 Cells.- Neuronal Transfection Using Particle-Mediated Gene Transfer.- Analysis of Gene Expression in Genetically Labeled Single Cells.- Immunocytochemistry and In Situ Hybridization: Their Combinations for Cytofunctional Approaches of Central and Peripheral Neurons.- In Situ Reverse Transcription PCR for Detection of mRNA in the CNS.- Immunocytochemical Labeling Methods and Related Techniques for Ultrastructural Analysis of Neuronal Connectivity.- Combined Electrophysiological and Morphological Analyses of CNS Neurons.- Tract Tracing Methods at the Light Microscopic Level.- Tract Tracing Methods at the Ultrastructural Level.- In Vivo Analysis of Cell Proliferation and Apoptosis in the CNS.- Confocal Imaging of Nerve Cells and Their Connections.- Confocal Imaging of Calcium Signaling in Cells from Acute Brain Slices.
