PCR (polymerase chain reaction) is now one of the most widely used of basic molecular biology techniques and is an indispensable research tool for the molecular biologist. The basic PCR technique provides the cornerstone for in vitro DNA amplification - allowing the researcher to produce large quantities of DNA from minute amounts of starting material.PCR: A Practical Approach Volume 2 is not a revised version of PCR: A Pracical
Approach, but sets out to address some of the exciting new applications fo PCR including cDNA cloning, mRNA quantitation and expression of proteins from PCR products, genomic DNA mapping and fingerprinting, and mutational analysis.
Rezensionen / Stimmen
This book gives a good insight into the more advanced PCR technology avaiable and will certainly be a useful addition to laboratories wishing to expand their PCR repertoire. * Dr Amanda Evans, Pharma Research, CIBA AG, Basel, Cell Biology International, 1996, Vol. 20, No. 6 *
Reihe
Sprache
Verlagsort
Zielgruppe
Illustrationen
3 colour halftones, halftones, line figures and tables
Maße
Höhe: 234 mm
Breite: 156 mm
Dicke: 20 mm
Gewicht
ISBN-13
978-0-19-963424-8 (9780199634248)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Klassifikation
Herausgeber*in
Department of Biochemistry
Department of Biochemistryboth at the University of Leeds
Regional DNA Laboratory, Leeds
1. Optimizing PCR ; 2. Use of specialty phosphoramidites in PCR ; 3. Chemical methods for 5' non-isotopic labelling of PCR probes and primers ; 4. Solid-phase PCR ; 5. Solid-phase sequencing of PCR products ; 6. cDNA cloning by RT - PCR ; 7. Quantification of DNA and RNA by PCR ; 8. PCR MIMICS: competitive DNA fragments for use in quantitative PCR ; 9. In vitro expression of proteins from PCR products ; 10. PCR-based approaches to human genome mapping ; 11. Fingerprinting of DNA and RNA using arbitrarily primed PCR ; 12. Mutational analysis ; 13. Mutational analysis: new mutations ; 14. Linear amplification for the in virto study of ligand/DNA interactions ; 15. Ligand-mediated PCR
