Growth factors play an important role in the regulation of proliferation and differentiation of specific cell types. This is a guide to studying growth factors, from the search for and characterization of new activities, to "in vivo" analysis and molecular biology approaches such as DNA cloning. Although cytokines is included in the list of growth factors, the book avoids protocols relating specifically to cytokines and this volume should be seen as complementary to the volume "Cytokines: A Practical Approach" in the same series.
Rezensionen / Stimmen
'for anyone planning to tackle these techniques ... this book is an invaluable source of hard-earned practical knowledge and experience from the front'
Louis C. Mahadevan, King's College, Trends in Cell Biology, Vol. 3, December 1993 ''this book is strongly recommended to anyone involved in growth-factor research'
C. Jane Robinson, National Institute for Biological Standards and Control, Potters Bar, TIBTECH, March 1994 (Vol. 12)
Reihe
Sprache
Verlagsort
Zielgruppe
Illustrationen
halftones, line drawings, tables
Maße
Höhe: 234 mm
Breite: 145 mm
Dicke: 16 mm
Gewicht
ISBN-13
978-0-19-963359-3 (9780199633593)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Klassifikation
Herausgeber*in
both at the Department of Experimental DermatologyLondon Hospital Medical College
1. Types of growth factor activity: detection and characterization of new growth factor activities ; 2. Detection of polypeptide growth factors: application of specific bio-assays and PCR technology ; 3. Induction of DNA synthesis and cell division in quiescent cells in response to small peptide growth factors ; 4. Novel gene transcription in response to growth factors: gene isolation by differential plaque hybridization ; 5. Embryological assays for growth factors ; 6. The role of TGF- in bone growth and bone repair ; 7. Localization of growth factor mRNA in tissue sections by in-situ hybridization ; 8. Detection and characterization of growth inhibitory factors: epidermal inhibitory pentapeptide (EIP) ; 9. Expression of growth factors in microbial systems ; 10. Immunoassays for growth factors ; 11. Determination of growth factor receptor distribution by immunocytochemistry or autoradiography and quantitation by ligand binding or FACS analysis ; Appendices
