Calcium is arguably the most diverse and widespread of the intra cellular second messengers, being involved in many mechanisms of cellular biochemistry, physiology, and pathiology, where it plays a key role in signal transduction. Hormones and other extracellular agents bring about many of their effects by causing changes intracellular calcium concentration. This book provides a comprehensive description of current techniques for measuring intracellular calcium concentrations, including protocols for the use of fluorescent, bioluminescent and metallochromic indicators, and various other intracellular probes. Also outlined are methods for controlling and changing the calcium ion concentration in cells, as well as procedures used to study the important proteins involved in the membrane tranport and intracellular binding of calcium. All of the chapters provide expert practical guidance in the techniques described and list any problems likely to be encountered. This book should appeal to a wide range of biological and biomedical researchers, particularly those involved in cellular, molecular and neuro-biology.
Sprache
Verlagsort
Zielgruppe
Für höhere Schule und Studium
Für Beruf und Forschung
Illustrationen
2 colour plates, 1 halftone, 84 line drawings, 12 tables, bibliography
ISBN-13
978-0-19-963131-5 (9780199631315)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Klassifikation
A.P. Thomas & F. Delaville: Use of fluorescent indicators for measurements of cytosolic free calcium in cell populations and single cells; P.H. Cobbold & J.A.C. Lee: Aequorin measurements of cytoplasmic free calcium; C.H. Orchard, M.R. Boyett, M. Hunter & C.H. Fry: Use of electrodes to study cellular Ca metabolism; M.V. Thomas: Metallochromic indicators; J.C. Metcalfe & G.A. Smith: NMR measurement of cytoplasmic free calcium concentration by fluorine labelled indicators; M.F. Wendt-Galliltelli & G. Isenberg: X-ray microanalysis; A. Fabiato: Ca buffering: computer programs and simulations; C.C. Ashley, P.J. Griffiths, T.J. Lea, P. Mulligan & R. Palmer: Use of fluorescent TnC derivatives and "caged" compounds to study cellular Ca phenomena; A.R. Means, I.C. Bagchi, M.F.A. Vanberkum & C.D> Rasmussen: Calmodulin; C.M. Boustead & J.H. Walker: Annexins: calcium-dependant phospholipid-binding proteins; B.A. Kenney, A.T. Kilpatrick & M. Spedding: Quantification of drugs acting at the calcium channel; J.P. Reeves: Sodium-calcium exchange activity in plasma membrane vesicles; E. Carafoli: The Ca-ATPase of the plasma membrane; S.L. Hamilton & C.A. Tate: Proteins involved in the uptake and release of Ca from the sarcoplasmic reticulum; J.G. McCormack & M. Crompton: Role and study of mammalian mitochondrial Ca transport and matrix Ca; D.A. Callaham & P.K. Hepler: Measurement of free calcium in plant cells.
