Flow cytometry and laser scanning cytometry are increasingly used in clinical and research settings, following improvements in instrument design and computing power and the increased availability of fluorescent agents. This book provides a comprehensive introduction to the theory and clinical applications of these techniques in the assessment of cell phenotype and function. With an emphasis on clinical relevance, the book presents the principles and potential of cytometry in the analysis of phenomena including cell-mediated cytotoxicity, metabolic burst, phagocytosis, cell-cell aggregation, receptor shedding and apoptosis. Guidance is given on data interpretation, quality control procedures, pitfalls and problems. All chapters contain detailed protocols from leading authorities in the field with extensive practical experience.
Rezensionen / Stimmen
'... an up-to-date book that is useful for everybody working in the cytometry field, as well as for those with a more general interest in biomedical sciences.' Rafael Nunez, Nature Cell Biology
Sprache
Verlagsort
Zielgruppe
Illustrationen
62 Tables, unspecified; 13 Plates, color; 3 Halftones, unspecified; 122 Line drawings, unspecified
Maße
Höhe: 254 mm
Breite: 193 mm
Dicke: 28 mm
Gewicht
ISBN-13
978-0-521-66029-7 (9780521660297)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Klassifikation
Herausgeber*in
Queen Mary University of London
1. Principles of flow cytometry Marion G. Macey; 2. Introduction to the general principles of sample preparation Desmond A. McCarthy; 3. Fluorescence and fluorochromes Desmond A. McCarthy; 4. Quality control in flow cytometry David Barnett and John T. Reilly; 5. Data analysis in flow cytometry Mark W. Lowdell; 6. Laser scanning cytometry: application to the immunophenotyping of hematological malignancies Richard J. Clatch; 7. Leucocyte immunobiology Marion G. Macey and Timothy M. Milne; 8. Immunophenotypic analysis of leucocytes in disease Jamie D. Cavenagh and Marion G. Macey; 9. Analysis and isolation of minor cell populations Terry Hoy; 10. Cell cycle, DNA and DNA ploidy analysis Paul D. Allen and Adrian C. Newland; 11. Cell viability, necrosis and apoptosis Paul D. Allen and Adrian C. Newland; 12. Phagocyte biology and function Marion G. Macey; 13. Intracellular measures of signalling pathways James W. Jacobberger and David W. Hedley; 14. Cell-cell interactions Marion G. Macey and Mary R. Cahill; 15. Nucleic acids Charles L. Goolsby; 16. Microbial infections David Lloyd, David J. Mason and Marc T. E. Suller; 17. Leucocyte cell surface antigens Marion G. Macey; 18. Recent and future developments: conclusions Desmond A. McCarthy; Appendix 1. Abbreviations; Appendix 2. Useful Internet sites.
