While originally being used for fluorogenic nucleic acid probes with a stem-loop structure, molecular beacon now refers to a whole family of fluorescence-up probes. This book intends to show a diverse set of instructive examples, from probe design to applications in clinical settings, guided by experts in the field who provide easy-to-follow experiments. The book commences with an introduction to the basic principles of fluorescence, and shows a diverse set of instructive examples, from probe design to applications in clinical settings, molecular beacon technology, the identification of transgenic mice, along with the potential of realtime PCR. This book summarizes all the latest findings and explores powerful techniques designed for geneticist, biotechnologists, molecular and cellular biologists, researchers and developers.
Reihe
Auflage
Softcover reprint of hardcover 1st ed. 2008
Sprache
Verlagsort
Zielgruppe
Für Beruf und Forschung
Research
Produkt-Hinweis
Broschur/Paperback
Klebebindung
Illustrationen
60
17 farbige Abbildungen, 60 s/w Abbildungen
XII, 266 p. 77 illus., 17 illus. in color.
Maße
Höhe: 229 mm
Breite: 152 mm
Dicke: 15 mm
Gewicht
ISBN-13
978-1-61737-726-6 (9781617377266)
DOI
10.1007/978-1-60327-040-3
Schweitzer Klassifikation
Basic Principles of Fluorescence and Energy Transfer.- Applications.- Multiplex Detection of Mutations.- Detection of Salmonella Species in Foodstuffs.- Identification of Homozygous Transgenic Mice by Genomic Real-Time PCR.- Quantitative Analysis of Gene Expression Relative to 18S rRNA in Carcinoma Samples Using the LightCycler® Instrument and a SYBR GreenI-based Assay: Determining FAT10 mRNA Levels in Hepatocellular Carcinoma.- Absolute Quantitation of Feline Leukemia Virus Proviral DNA and Viral RNA Loads by TaqMan® Real-time PCR and RT-PCR.- High-Throughput Real-Time PCR.- Design and Use of Scorpions Fluorescent Signaling Molecules.- Hybridization Probe Pairs and Single-Labeled Probes: an Alternative Approach for Genotyping and Quantification.- New Technologies.- EasyBeaconsT for the Detection of Methylation Status of Single CpG Duplets.- Quenched Autoligation Probes.- HyBeacon® Probes for Rapid DNA Sequence Detection and Allele Discrimination.- FIT-Probes in Real-Time PCR.- SNP Genotyping by Unlabeled Probe Melting Analysis.- Non-Nucleic Acid Targets.- Molecular Beacons for Protein-DNA Interaction Studies.- DNA Polymerase Profiling.- Modular Reporter Hairpin Ribozymes for Analyzing Molecular Interactions.- Screening of Molecular Interactions Using Reporter Hammerhead Ribozymes.
