Chapter 1. Application of In Situ Hybridization to the Study of Rhythmic Neural Systems /H. Elliott Albers, R. Thomas Zoeller, and Kim L. Huhman -- Chapter 2. Estimation of the mRNAs Encoding the Cholinergic Muscarinic Receptor and Acetylcholine Vesicular Transport Proteins Involved in Central Cardiovascular Regulation /Jerry J. Buccafusco, Lu C. Zhang, and Mark A. Prendergast -- Chapter 3. Voltammetric Detection of Nitric Oxide (NO) in the Rat Brain: Release Throughout the Sleep-Wake Cycle /Sophie Burlet and Raymond Cespuglio -- Chapter 4. Sleep Regulatory Substances: Change in mRNA Expression Linked to Sleep /Zutang Chen and James M. Krueger -- Chapter 5. Immediate Early Genes as a Tool to Understand the Regulation of the Sleep-Waking Cycle: Immunocytochemistry, In Situ Hybridization, and Antisense Approaches /Chiara Cirelli, Maria Pompeiano, and Giulio Tononi -- Chapter 6. Methods for the Measurement of Adenylyl Cyclase Activity /Charles W. Emala -- Chapter 7. Methods Used to Assess Specific Messenger RNA Expression During Sleep /Mary Ann Greco, Lalini Ramanathan, Radhika Basheer, and Priyattam J. Shiromani -- Chapter 8. Competition Binding Assays for Determining the Affinity and Number of Muscarinic Receptor Subtypes in Tissue Homogenates /A. Urban Hoglund and Helen A. Baghdoyan -- Chapter 9. Isolation and Identification of Specific Transcripts by Subtractive Hybridization /Thomas S. Kilduff, Luis de Lecea, Hiroshi Usui, and J. Gregor Sutcliffe -- Chapter 10. Use of In Situ Hybridization Histochemistry to Study Muscarinic Receptor mRNA Expression in Brains of Sleep-Deprived Rats /Clete Kushida and Donna M. Simmons -- Chapter 11. Transcriptional Regulation of Putative Sleep-Promoting Compounds /Miroslaw Mackiewicz, Sigrid C. Veasey, and Allan I. Pack -- Chapter 12. Chemical Mutagenesis and Screening for Mouse Mutations with an Altered Rest-Activity Pattern /Patrick M. Nolan, Thomas A. Houpt, and Maja Bucan -- Chapter 13. Reverse Transcription mRNA Differential Display: A Systematic Molecular Approach to Identify Changes in Gene Expression Across the Sleep-Waking Cycle /Maria Pompeiano, Chiara Cirelli, and Giulio Tononi -- Chapter 14. In Situ Hybridization of Messenger RNA in Sleep Research /Tarja Porkka-Heiskanen, Jussi Toppila, and Dag Stenberg -- Chapter 15. New Directions in the Analysis of Brain Substances Related to Sleep and Wakefulness /Gary Siuzdak and Steven Henriksen -- Chapter 16. Sleep and Circadian Rest-Activity Rhythms in Prion Protein Knockout Mice /Irene M. Tobler; Marek Fischer; and Jean C. Manson -- Chapter 17. Measurement of Nitric Oxide in the Brain Using the Hemoglobin Trapping Technique Coupled with In Vivo Microdialysis /Julie A. Williams, Steven R. Vincent, and Peter B. Reiner -- Chapter 18. Mapping Regional Cerebral Protein Synthesis During Sleep /Rebecca K. Zoltoski -- Index.
