Antisense technology is a powerful procedure that permits the controlled silencing of a specific gene for investigations of mRNA and protein function. This valuable text provides proven step-by-step protocols for antisense techniques in a range of different organisms and cell culture systems. In addition it discusses the potential benefits and problems for various antisense methods which complement gene knock-out experiments. The book includes antisense techniques
such as: analysis of nucleic acid structures; measurement and evaluation of antisense effects; selection, preparation and the use of antisense oligonucleotides; in vitro RNA transcription; construction strategies, testing and optimization of catalytic antisense RNAs based on hammerhead ribozymes;
synthesis and evaluation of 2-5 A- antisense chimeras for targeted degradation of RNA. The application of these technologies are then described, with chapters on antisense techniques in Dictyostelium, plants and the medical uses and benefits of antisense sense technology both in vitro and in vivo. Finally, non-antisense effects of oligonucleotides, "anti-sense rescue", shotgun antisense mutagenesis, and in vivo, in vitro and in situ
detection of sense:antisense duplexes by structure-specific anti-RNA antibodies are discussed. The numerous hints and tips for success and advice on trouble shooting, make this authoritative text invaluable to all those researchers who work on gene expression, RNA transcription, or protein function.
Rezensionen / Stimmen
Genetic researchers will find ANTISENSE TECHNOLOGIES a most valuable source of information. * Library of Science, Winter 1998 * providing clear and precisely described protocols which cover a broad range of applications.The experience gained from experimental and clinical trials in the antisense area has been concentrated in this book which provides updated data and methodologies that may be extremely useful for everybody who is already familiar with antisense oligonucleotides and for those investigators who are approaching this field in continuous progress for the first time. * FEBS Letters 434 (1998) 442-446 * '...this volume reaches the standard we have come to expect from the Practical Approach series. The standard of presentation reflects the expertise of the authors and the protocol are described clearly with useful practical notes.' * Glenn Matthews, SGM Quarterly Nov. 1998. *
Reihe
Sprache
Verlagsort
Zielgruppe
Illustrationen
halftones, line figures, tables
Maße
Höhe: 235 mm
Breite: 157 mm
Dicke: 19 mm
Gewicht
ISBN-13
978-0-19-963583-2 (9780199635832)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Klassifikation
Herausgeber*in
Professor, School of Biological SciencesQueen Mary and Westfield College, London
Abteilung Genetik FB19Universitat Kassel, Kassel
1. Nucleic acid structures ; 2. Evaluation of antisense effects ; 3. Selecting, preparing, and handling antisense oligodeoxyribonucleotides ; 4. In vitro RNAs ; 5. Catalytic antisense RNA based on hammerhead ribozymes ; 6. 2-5 A-Antisense chimaeras for targeted degradation of RNA ; 7. In vitro applications of antisense RNA and DNA ; 8. Antisense applications in Dictyostelium: a lower eukaryotic model system ; 9. Applications of antisense technology in plants ; 10. Antisense molecules and ribozymes: medical applications ; 11. Non-antisense effects of oligodeoxynucleotides ; 12. Antisense rescue ; 13. Shotgun antisense mutants ; 14. Detection of sense-antisense duplexes by structure-specific anti-RNA antibodies
