Cancer Cell Culture

Part I. Introduction to Cancer Cell Culture

Basic Principles of Cancer Cell Culture
Simon P. Langdon

Essential Techniques of Cancer Cell Culture
Kenneth G. Macleod and Simon P. Langdon

Part II. Characterization and Authentication

Characterization and Authentication of Cancer Cell Lines: An Overview
Simon P. Langdon

Authentication of Cancer Cell Lines by DNA Fingerprinting
Wilhelm G. Dirks and Hans G. Drexler

Cytogenetic Characterization of Tumor Cell Lines
Roderick A. F. MacLeod and Hans G. Drexler

Part III. Isolation and Culture of Specialized Cell Types

Isolation and Culture of Colon Cancer Cell Lines
Jae-Gahb Park, Ja-Lok Ku, and So-Yeon Park

Isolation and Culture of Melanoma Cell Lines
Adam I. Riker

Isolation and Culture of Human Brain Tumor Cells
German G. Gomez and Carol A. Kruse

Isolation and Culture of Renal Cancer Cell Lines
Jae-Gahb Park, Ja-Lok Ku, and So-Yeon Park

Isolation and Culture of Prostate Cancer Cell Lines
Nora M. Navone, Matilde Olive, and Patricia Troncoso

Isolation and Culture of Ovarian Cancer Cell Lines
Simon P. Langdon

Isolation and Culture of Leukemia Cell Lines
Hans G. Drexler

Part IV. Functional Assays in Cell Culture

Cell Sensitivity Assays: Clonogenic Assay
Jane A. Plumb

Cell Sensitivity Assays: The MTT Assay
Jane A. Plumb

PARP Cleavage as a Means of Assessing Apoptosis
Peter Mullen

Detection of Apoptosis by the TUNEL Assay
John Lawry

Apoptosis Measurement by Annexin V Staining
Eric Miller

Cell Adhesion Assays
William N. Scott

Radial Monolayer Cell Migration Assay
Michael E. Berens and Christian Beaudry

Invasion and Motility Assays
William N. Scott

Identification of Senescence in Cancer Cells
Lesley J. Jardine

Angiogenesis Assays
William N. Scott

Flow Cytometric DNA Analysis of Human Cancer Cell Lines
Peter Mullen

Part V. Modification of Cancer Cells

DNA-Mediated Gene Transfer
Amanda J. M. O'Donnell

Development of Drug-Resistant Models
Helen M. Coley

Immortalization of Human Prostate Cells With the Human Papillomavirus Type 16 E6 Gene
Norman J. Maitland, Catherine A. Macintosh, Christian Schmitz, and Shona H. Lang

The Use of Matrigel to Facilitate the Establishment of Human Cancer Cell Lines as Xenografts
Peter Mullen

Part VI. Coculture Systems

Coculture of Prostate Cancer Cells
Shona H. Lang and Norman J. Maitland

Coculture of Ovarian Cells Using Porous Tissue Culture Inserts
Katherine E. Snow and Simon P. Langdon

Part VII. Cell Line Contamination

Cell Culture Contamination: An Overview
Simon P. Langdon

Detecting Mycoplasma Contamination in Cell Cultures by Polymerase Chain Reaction
Cord C. Uphoff and Hans G. Drexler

Elimination of Mycoplasma from Infected Cell Lines Using Antibiotics
Cord C. Uphoff and Hans G. Drexler

Appendix 1: Formulations of Commonly Used Cell Culture Media

Appendix 2: Human Cancer Cell Lines Available from the ATCC and DSMZ Cell Banks (Combined List)

Index