`In the second edition of Principles I have attempted to maintain the emphasis on basics, while updating the examples to include more recent results from the literature. There is a new chapter providing an overview of extrinisic fluorophores. The discussion of timeresolved measurements has been expanded to two chapters. Quenching has also been expanded in two chapters. Energy transfer and anisotropy have each been expanded to three chapters. There is also a new chapter on fluorescence sensing. To enhance the usefulness of this book as a textbook, most chapters are followed by a set of problems. Sections which describe advanced topics are indicated as such, to allow these sections to be skipped in an introduction course. Glossaries are provided for commonly used acronyms and mathematical symbols. For those wanting additional informtion, the final appendix contains a list of recommended books which expand on various specialized topics.'
from the author's Preface
Auflage
Sprache
Verlagsort
Verlagsgruppe
Kluwer Academic Publishers Group
Zielgruppe
Für höhere Schule und Studium
Für Beruf und Forschung
Editions-Typ
Illustrationen
Maße
Gewicht
ISBN-13
978-0-306-46093-7 (9780306460937)
DOI
10.1007/978-1-4757-3061-6
Schweitzer Klassifikation
Dr. J.R. Lakowicz is Professor of Biochemistry at the University of Maryland School of Medicine and Director of the Center for Fluorescence Spectroscopy. Dr. Lakowicz has published over 400 scientific articles, has edited numerous books, holds 16 issued patents, and is the sole author of the widely used text, Principles of Fluorescence Spectroscopy, also published by Kluwer Academic/Plenum Publishers, now in its Second Edition.
1. Introduction to Fluorescence. 2. Instrumentation for Fluorescence Spectroscopy. 3. Fluorophores. 4. Time-Domain Lifetime Measurements. 5. Frequency-Domain Lifetime Measurements. 6. Solvent Effects on Emission Spectra. 7. Dynamics of Solvent and Spectral Relaxation. 8. Quenching of Fluorescence. 9. Advanced Topics in Fluorescence Quenching. 10. Fluorescence Anisotropy. 11. Time-Dependent Anisotropy Decays. 12. Advanced Anisotropy Concepts. 13. Energy Transfer. 14. Time-Resolved Energy Transfer and Conformational Distributions of Biopolymers. 15. Energy Transfer to Multiple Acceptors in One, Two or Three-Dimensions. 16. Protein Fluorescence. 17. Time-Resolved Protein Fluorescence. 18. Excited State Reactions. 19. Fluorescence Sensing. 20. Long Lifetime Metal-Ligand Complexes. 21. DNA Technology. 22. Phase Sensitive and Phase Resolved Emission Spectra. Appendixes: I. Corrected Emission Spectra. II. Fluorescent Lifetime Standards. III. Additional Reading Index
