Large-Scale Protein Purification: Introduction ; Strategies for Large-Scale Protein Purification ; Separations in Biotechnology: The Key Role of Adsorptive Separations ; Peptide and Protein Partitioning in Aqueous Two-Phase Systems: Effect of Amino Acid Sequence ; Protein Separation via Polyelectrolyte Complexation ; Mechanisms of Protein Retention in Hydrophobic Interaction Chromatography ; Anion Exchange Stationary Phase for *b-Galactosidase, Bovine Serum Albumin, and Insulin: Separation and Sorption Characteristics ; Radial-Flow Affinity Chromatography for Trypsin Purification ; Impact of Continuous Affinity-Recycle Extraction (CARE) in Downstream Processing ; Novel Metal-Affinity Protein Separations ; Recovery of Recombinant Proteins by Immunoaffinity Chromatography ; Chelating Peptide-Immobilized Metal-Ion Affinity Chromatography ; Site-Specific Proteolysis of Fusion Proteins ; Purification Alternatives for IgM (Human) Monoclonal Antibodies ; Analytical, Preparative, and Large-Scale Zone Electrophoresis ; Applied Electric Fields for Downstream Processing