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This volume provides readers with an overview of lentiviral vector applications and includes detailed laboratory protocols relevant to neuroscience research. The chapters are organized into five sections: Section I provides an overview of lentiviral vector properties, including practical methods for vector preparation and delivery into the brains of rodents and non-human primates, as well as delivery into non-human primate embryos for transgenic animal production. Sections II to IV explore basic neuroscience covering anatomical, molecular and developmental, and behavioral and physiological neuroscience. Section V focuses on clinical neuroscience, offering protocols related to gene therapy for motor neuron and ocular diseases. Written in the highly successful Methods in Molecular Biology series format, each chapter includes an introduction to the topic, a list of required materials and reagents, step-by-step protocols that are easy to reproduce, and expert tips for troubleshooting and avoiding common pitfalls. Cutting-edge and thorough, Lentiviral Vectors: Application for Basic and Clinical Neuroscience is a valuable resource for any researcher looking to learn more about this important and advancing field.
Properties and Application of Pseudotyped Lentiviral Vectors.- Production and Purification of Lentiviral Vectors.- Lentiviral Vector Administration into the Mouse Brain.- Stereotaxic Injection of Lentiviral Vectors into the Primate Brain.- Optimized Lentiviral Vector Production Using Dual-Pseudotyping with VSV-G and Sendai Virus HN Glycoproteins for Enhanced Gene Delivery in Diverse Cell Types.- Preparation of Lentiviral Vectors for the Generation of Transgenic Cynomolgus Monkeys.- Ubiquitous and Brain Cell-Type-Specific Promoters and Enhancers in Lentiviral Vectors.- Efficient Labeling of Somatodendritic Membranes of the Central Neurons.- Visualization of Selective Neural Pathways by Double Viral Infection Method.- Lentivirus-Mediated Measurement of Transcription Factor Activity in the Brain.- Birth Date-Dependent Tracing of Neuronal Cell Lineage.- Application of Lentivirus Vectors to Adult Neurogenesis and Manipulation of Neural Stem Cells.- Direct Reprogramming of Microglia into Neurons.- Selective Neural Pathway Targeting by Using Lentiviral Vectors.- Synaptic Silencing with Transactivation of Tetanus Neurotoxin for Pathway-Selective Manipulation.- Optogenetic Approach for In Vivo Manipulation of Neural Circuits Using Lentiviral Vectors.- Selective Chemogenetic Blockade of Cerebello-Rubral Pathway by Double-Virus Infection Method Using Lentivirus and Adeno-Associated Virus in Rats.- Development of a Gene Therapy with Lentiviral Vectors for Spinocerebellar Ataxias.- Lentiviral Vector-Mediated Gene Delivery for iPSC-Related Neuroscience Research.- Phase 1/2a Clinical Trial Protocol for Lentiviral Vector-Based Retinal Gene Therapy to Slow the Progression of Retinitis Pigmentosa.- CAR-T Cell Therapy for Central Nervous System Tumors.
