DNA Probes

Part 1 Background: molecular hybridization theory and history; some general considerations; types of applications. Part 2 Sample preparation: extraction of DNA from blood; extraction of DNA from tissues; extraction of RNA; preparations of slides for "in situ" hybridization; extraction of nucleic acid from stool, urine and other sources; other methods for isolating nucleic acids. Part 3 Isotope labeling procedures: nick translation; primer extension; SP6/T7 generated RNA probes; tailing and 3' labeling with terminal transferase; labeling with polynucleotide kinase; iodination of cytosine. Part 4 Non-isotopic labeling procedures: enzymatic modification; chemical modification; photoblotin; peroxldase; liner arm modification; ollgonucleotide probes. Part 5 Hybridization formats and detection procedures: filter hybridization; in situ hybridization; soution hybridization; sandwich hybridization; other formats. Part 6 Amplification systems: polymerase chain reaction; QB; TAS; Prove networks. Appendices: buffers; color reagents; suppliers.