Functional Genomics

Prediction of Protein Tertiary Structures using MUFOLD.-Prediction of Protein Functions.-Genome Wide Screens for Expressed Hypothetical Proteins.-Self Custom-Made SFP Arrays for Non-Model Organisms.-Construction and Analysis of Full-length and Normalized cDNA Libraries from Citrus.-Assembling Linear DNA Templates for in vitro Transcription and Translation.-Automated Computational Analysis of Genome-wide DNA Methylation Profiling Data from HELP-tagging Assays.-Detection of RNA Editing Events in Human Cells using High- Throughput Sequencing.-Comparative Study of Differential Gene Expression in Closely Related Bacterial Species by Comparative Hybridization.-Whole Genome RT-qPCR MicroRNA Expression Profiling.-Using Quantitative Real-Time Reverse Transcriptase Polymerase Chain Reaction to Validate Gene Regulation by PTTG.-FRET-Based Real-Time DNA Microarrays.- 2-D Gel Electrophoresis: Constructing 2D-Gel Proteome Reference Maps.-The use of Antigen Microarrays in Antibody Profiling.-Limited Proteolysis in Proteomics using Protease-immobilized Microreactors.-Mass Spectrometry for Protein Quantification in Biomarker Discovery.-High-throughput Microtitre Plate-based Assay for DNA Topoisomerases.-Microscale Thermophoresis as a Sensitive Method to Quantify Protein - Nucleic Acid Interactions in Solution.-Bioluminescence Resonance Energy Transfer: an Emerging Tool for the Detection of Protein-protein Interaction in Living Cells.-LuMPIS - Luciferase-based MBP-pull-down Protein Interaction Screening System.-Yeast Two-Hybrid Screens: Improvement of Array-based Screening Results by N- and C-terminally Tagged Fusion Proteins.-Inducible microRNA-mediated Knockdown of the Endogenous Human Lamin A/C Gene.- Multiple-gene Silencing using Antisense RNAs in Escherichia coli.-Functional Screen of Zebrafish Deubiquitylating Enzymes by Morpholino Knockdown and in situ Hybridization.-Silencing of Gene Expression by GymnoticDelivery of Antisense Oligonucleotides.-Polycistronic Expression of Interfering RNAs from RNA Polymerase III Promoters.-Metabolite Analysis of Cannabis sativa L. by NMR Spectroscopy.-Metabolome Analysis of Gram-positive Bacteria such as Staphylococcus aureus by GC-MS and LC-MS.-Metabolic Fingerprinting Using Comprehensive Two-dimensional Gas Chromatography - Time-of-flight Mass Spectrometry (GC×GC-TOF-MS).