Signal Transduction Immunohistochemistry

Overview of the Generation, Validation, and Application of Phosphosite-Specific Antibodies.- Selection and Validation of Antibodies for Signal Transduction Immunohistochemistry.- An Overview of Western Blotting for Determining Antibody Specificities for Immunohistochemistry.- Optimized Protocol to Make Phospho-Specific Antibodies that Work.- Methodology and Technology for Stabilization of Specific States of Signal Transduction Proteins.- An Enhanced Antigen Retrieval Protocol for Immunohistochemical Staining of Formalin-Fixed, Paraffin-Embedded Tissues.- Imaging Techniques in Signal Transduction IHC.- Practical Considerations of Image Analysis and Quantification of Signal Transduction IHC Staining.- Flow Cytometric Analysis of Cell Signaling Proteins.- CytoSys: A Tool for Extracting Cell Cycle-Related Expression Dynamics from Static Data.- Signaling Events Initiated by Kappa Opioid Receptor Activation: Quantification and Immunolocalization Using Phospho-Selective KOR, p38-MAPK, and K_IR 3.1 Antibodies.- Immunohistochemical Assessment of Signal Transduction and Cell-Cycle Networks in Neural Tumors.- Novel Multi-Color Immunofluorescence Technique Using Primary Antibodies Raised in the Same Host Species.- Activation and Differentiation of Mesenchymal Stem Cells.- Double In situ Detection of Sonic Hedgehog mRNA and pMAPK Protein in Examining the Cell Proliferation Signaling Pathway in Mouse Embryo.- Identifying Intracellular Sites of Eicosanoid Lipid Mediator Synthesis with EicosaCell Assays.- Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads.