While for many years NMR spectroscopy was one of the most useful methods of analyzing smaller molecules, nowadays it is an indispensable tool in determining the structure of complex biomolecules too. One reason for this is not least the development of increasingly powerful appliances that enabled this complicated analysis using tiny amounts of the substances in the first place. Just as the image of a 2D spectrum allows an insight into the spatial structure of a molecule, the chronological examination of an NMR experiment enables the description of the molecular dynamics. It is only by explaining such characteristics that we can really come to understand the functioning of biomolecules.
This book is neither a purely analytical nor bioorganic work, and as such fills a gap for everybody working in determining the structure of carbohydrates and their conjugates. In a clearly comprehensible manner, the text summarizes the latest knowledge surrounding the use of NMR spectroscopy with such biologically important substance classes as polysaccharides, glycoproteins and glycolipides. Furthermore, it provides an insight into the interactions with other biomolecules such as proteins, nucleic acids or carbohydrates. A valuable guide for all glucochemists.
Auflage
Sprache
Verlagsort
Zielgruppe
Für höhere Schule und Studium
Für Beruf und Forschung
Spektroskopiker, Chemiker, Biochemiker, Medizinische Chemiker, Pharmazeutische Chemiker
Illustrationen
320
31 s/w Tabellen, 4 farbige Abbildungen, 320 s/w Abbildungen
Illustrations
Maße
Höhe: 24 cm
Breite: 17 cm
Dicke: 20 mm
Gewicht
ISBN-13
978-3-527-30414-1 (9783527304141)
Schweitzer Klassifikation
BIOLOGICAL SIGNIFICANCE OF GLYCOSTRUCTURES
The impact of NMR in understanding their structure and function
NMR OF GLYCOCONJUGATES
NMR of glycoproteins. The NMR view of the impact of the saccharide chain in the structure, dynamics and function
NMR of glycolipids. The use of media other than water. Orientation in lipophilic media. The use and impact of residual dipolar couplings
NMR OF OLIGO- AND POLYSACCHARIDES
Conformational analysis of saccharides and NMR experimental evidence. The general approach. Advantages and problems of the application of NMR methods
The use of NMR to understand the molecular motional features of saccharides. NMR relaxation parameters and dynamics. Global and internal motions
NMR of sulfated oligo- and polysaccharides. The building blocks: monosaccharide conformation. The glycosidic linkages. The influence of the charges and counterions. From oligo- to polysaccharides. Anisotropy of motion
Activated sugars. The general approach to understand their conformation by NMR
Applications of homo- and heteronuclear coupling constants to conformational analysis of pyranoses and furanoses. Use and benefit of isotope labelling. The experimental approach. Karplus-like relationships and C-H and C-C couplings /
Residual dipolar couplings in bacterial polysaccharides. Orientation in diluted liquid crystals. Use and benefit of isotope labelling. The experimental approach. Orientation, conformation and dynamics
BIOMOLECULAR INTERACTIONS
Carbohydrate-protein interactions investigated by NMR STD. The experimental approach. Epitope mapping of oligosaccharides with STD. Detection of bio-active compounds with STD Transfer NOE. The experimental approach. Applications of TR-NOE to detect protein carbohydrate interactions. Detecting binding. The conformation of the bound state. Use and benefit of isotope labelling for TR-NOE experiments
Carbohydrate-carbohydrate interactions investigated by NMR. The experimental approach
Carbohydrate-nucleic acid interactions investigated by NMR. The experimental approach. Aminoglycoside-RNA interactions
EXPERIMENTS: TECHNIQUES AND APPLICATION
Selective nD experiments. The heteronuclear and homonuclear building blocks
Detection of hydroxyl protons. The problem of fast exchange and the ways of circumvention. The use of solvents other than water. Experimental techniques
New NMR techniques. The state-of-the-art pulse sequences and their impact for glyco NMR. Improving sensitivity.
The new technologies. The use of cryo probe technology and its impact for glyco NMR
