Molecular techniques are now being widely applied in clinical pathology laboratories, yet many people working in these facilities have only limited experience of such procedures. This work, together with Volume I, brings together a broad spectrum of protocols relevant to the routine molecular analysis of pathological samples. Volume I featured those methods which relate to morphological analysis, such as immunocytochemistry, in situ hybridization, chromosome analysis and flow cytometry. This second volume demonstrates additional applications of molecular techniques to clinical samples, including the use of PCR, DNA sequencing and nucleic acid hybridization for such diverse purposes as pathogen identification and forensic studies. For all the protocols, guidance and instructions are given on how to ensure accuracy and reliability, which should appeal to people working at all levels in apthology. The two volumes are also available as a set.
Sprache
Verlagsort
Zielgruppe
Für höhere Schule und Studium
Für Beruf und Forschung
Illustrationen
halftones, line figures, tables, bibliography
ISBN-13
978-0-19-963239-8 (9780199632398)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Klassifikation
Extraction of nucleic acids from clinical samples and cultured cells, V.T-W. Chan; probe preparation and labelling, J.E. Stickland; molecular hybridization of nucleic acids, V.T-W. Chan; the polymerase chain reaction and the molecular genetic analysis of tissue biopsies, D.K. Shibata; DNA sequencing, M.B. Llewelyn; determination of genital human papillomavirus infection of cells and tissues by generic polymerase chain reaction amplification, H.M. Bauer and M.M. Manos; the polymerase chain reaction for human papillomavirus screening in diagnostic cytopathology of the cervix, J.M.M. Walboomers et al; molecular detection and identification of pathogenic organisms, B.J. McCready and J.A. Chimera; human identification by DNA analysis - the amplification fragment length polymorphism (AMPFLP) analysis technique, M. Eisenberg and J.A. Chimera; analysis of archival material - application to tumour pathology, W. Yasui et al.
