In HaemophilusiInfluenzae Protocols, leading research scientists and infectious disease specialists detail in a readily reproducible format the major molecular and immunological techniques for exploring the pathogenicity of H. influenzae. Described with step-by-instructions to ensure robust and successful experimental results, the techniques cover plasmid analysis, proteomics, genomics, DNA array technology, gene expression, mutagenesis (transposon and nontransposon), and structural analysis. These methods illuminate how the bacterium causes disease, as well as how best to develop novel vaccines and antibiotics against the organism.
Rezensionen / Stimmen
"This excellent book is recommended for investigators involved in laboratory research on H. influenzae from the beginning student to the experienced investigator. The book contains abundant, detailed protocols and practical tips that will be useful for all investigators." - Clinical Infectious Diseases
"...an excellent source book and an inspiring reading." - International Journal of Medical Microbiology
"In addition to detailed information provided in the individual chapters, the book is also valuable by providing an extensive list of 841 references....there are not many microbiological books of such high quality. The authors present highly specialized information in a comprehensible and friendly way." -Folia Microbioligica
Reihe
Auflage
1st ed. Softcover of orig. ed. 2003
Sprache
Verlagsort
Zielgruppe
Für Beruf und Forschung
Research
Illustrationen
Maße
Höhe: 235 mm
Breite: 155 mm
Dicke: 19 mm
Gewicht
ISBN-13
978-1-61737-255-1 (9781617372551)
DOI
Schweitzer Klassifikation
The Pathogenesis of Disease Due to Nontypeable Haemophilus influenzae.- The Pathogenesis of Disease Due to Type b Haemophilus influenzae.- General Methods for Culturing Haemophilus influenzae.- Transformation of Haemophilus influenzae.- Diagnosis of Infection.- Characterization of Plasmids.- Protein Characterization by Two-Dimensional Gel Electrophoresis.- Monitoring Gene Expression Using DNA Arrays.- Gene Expression Technology.- The Genome Sequence of Haemophilus influenzae.- Structural Profiling of Short-Chain Lipopolysaccharides from Haemophilus influenzae.- Mutagenesis of H. influenzae.- Transposon Tn 10.- In Vivo Expression of Bacterial Genes During Human Infections.- ELISA.- Opsonophagocytosis Assay Using Flow-Cytometry.- In Vitro Models of Infection I-Human Respiratory Tissue Organ Culture.- In Vitro Models of Infection II-Human Umbilical Vein Endothelial Cells (HUVECs) System.- Animal Models.
