This book presents a practical guide to the laboratory techniques commonly used in the analysis of lipids. It covers methods of lipid extraction in solvent and solid phase systems, as well as methods of derivation formation needed for a number of analytical procedures such as gas liquid chromatography and mass spectrometry. The book also describes in practical detail the techniques for complete analysis of both isolated lipids and lipid mixtures. These include GLC, HPLC, TLC and NMR. These molecules are found in every living system, and are of interest to the chemical, biotechnological and pharmaceutical industries in addition to academic research workers.
Sprache
Verlagsort
Zielgruppe
Für höhere Schule und Studium
Für Beruf und Forschung
Illustrationen
halftones, line drawings, tables, bibliography
ISBN-13
978-0-19-963098-1 (9780199630981)
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Schweitzer Klassifikation
Part 1 Introduction: fatty acids; other monofunctional compounds; lipids; acylglycerols (glycerides); phospholipids; waxes; ether lipids; glycosyldiacylglycerols; sterol esters; sphingolipids; Frank D. Gunstone. Part 2 Extraction of lipids and derivative formation: preliminary treatment of tissues; treatment of samples; derivative formation; isolation (extraction) of volatiles; Shiela Hamilton, et al. Part 3 Thin-layer chromatography: materials and apparatus; application of sample and development of plates; TLC on standard silica; TLC on modified silica; detection systems; quantitation; TLC on coated quartz micro-columns; R. James Henderson and Douglas R. Tocher. Part 4 Gas chromatography of lipids: applications of GC analysis of lipids; basic principles; basic instrumentation; GC column detection; compound identification; applications; trouble-shooting; Richard P. Evershed. Part 5 High-performance liquid chromatography: theory of HPLC; mechanisms of separation; equipment; fractionation into lipid classes; separation of lipid classes; separation of fatty acids; separation of glycerolipids; separation of phospholipids; separation of cholesterol and cholesterol esters; Peter A. Sewell. Part 6 Radiotracers in lipid analysis: measurement of labelled compounds; radiochromatography; radioisotope dilution analysis and radioimmunoassay; radiochemical purity; availability of radiolipids and related compounds; health and safety; Colin G. Taylor. Part 7 High-resolution 1H and 13C NMR: 1H NMR spectroscopy; 13C NMR spectroscopy; Frank D. Gunstone. Part 8 Mass spectroscopy of lipids: basic principles; basic instrumentation; applications; Richard P. Evershed. Appendix: suppliers of specialist items
