Gene probes find a wide variety of uses in molecular biology, and are essential tools for genetic research. This volume describes some of their many applications, from the study of nucleic acids, through blotting techniques and gene targeting, to analysis of human genetic mutations. A Companion volume, Genes Probes 1, covers preparation of the probes. The two books are timely additions to the series, as this is a progressive field which continues to grow in
importance.
Reihe
Sprache
Verlagsort
Zielgruppe
Illustrationen
halftones, line figures, tables
Maße
Höhe: 234 mm
Breite: 156 mm
Dicke: 23 mm
Gewicht
ISBN-13
978-0-19-963414-9 (9780199634149)
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Schweitzer Klassifikation
Herausgeber*in
both at the Department of Biochemistry and Molecular BiologyUniversity of Leeds
1. Hybridization strategy ; 2. Screening recombinant DNA libraries by hybridization and amplification ; 3. Screening human recombinant libraries with nucleic acid probes ; 4. Antibody screening of bacterial expression systems ; 5. Isolation of cDNA clones by direct expression in mammalian cells ; 6. Genomic mapping and gene structure analysis ; 7. Analysis of gene expression by blotting techniques ; 8. In situ hybridization of nucleic acid probes to cellular RNA ; 9. Applications of chromosomal in situ hybridization ; 10. Analysis of gene expression analysis by PCR: a quantitative approach ; 11. Analysis of protein expression by immunocytochemistry ; 12. Gene targeting in mammalian cells ; 13. Directed mutagenesis and mutant analysis ; 14. Human gene mutational analysis ; Appendix 1 ; Appendix 2
