The preparation of gene probes is a core application in molecular biology creating essential tools for genetic research. This volume together with its forthcoming companion volume, Gene Probes 2, provides a timely and important resource for all researchers in this field. Gene Probes 1 focuses on the wide range of techniques now available for the purification, synthesis, labelling, detection, and quantitation of probes, including
antibody probes. Gene Probes 2 will concentrate on describing techniques for specific research goals.
Contents List: Strategies for using gene probes; Preparation of nucleic acids for use as probes; DNA radiolabelling and detection; Non-radioactive labelling of DNA probes; Preparation of oligodeoxynucleotide probes; Probe preparation by PCR; Synthesis of labelled riboprobes; Antibody probes
Rezensionen / Stimmen
This book is intended for use as a laboratory manual, and contains practical assistance to the best of the current methodology within its subject area. * International Journal of Biochemistry and Cell Biology, Vol. 28, No. 7, 1996 * a very practical book * Cellular & Molecular Biology, Vol. 42, No. 3, 1996 *
Reihe
Sprache
Verlagsort
Zielgruppe
Illustrationen
Maße
Höhe: 234 mm
Breite: 157 mm
Dicke: 16 mm
Gewicht
ISBN-13
978-0-19-963400-2 (9780199634002)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Klassifikation
Herausgeber*in
both at the Department of Biochemistry and Molecular BiologyUniversity of Leeds
Introduction ; 1. Strategies for using gene probes ; 2. Preparation of nucleic acids for use as probes ; 3. DNA radiolabelling and detection ; 4. Non-radioactive labelling of DNA probes ; 5. Preparation of synthetic oligodeoxynucleotide probes ; 6. Probe preparation of PCR ; 7. Synthesis of labelled riboprobes ; 8. Antibody probes ; Appendix 1 ; Appendix 2
