Part 1 Biosynthesis and processing: molecular mechanism of nucleocytoplasmic transport of proteins through the nuclear membrane; membrane and functional topography of cytochrome "b5"; microsomal aldehyde dehydrogenase in the endoplasmic reticulum; structure, function and intracellular localization of the proprotein-processing enzyme furin; peroxisome biogenesis and peroxisome assembly disorders; roles of cytosolic factors in mitochondrial protein import in higher animals; cytoplasmic chaperones determine the targetting pathway of precursor proteins to mitochrondria. Part 2 Membrane assembly and topogenic signals: translocational pausing and the regulation of membrane protein biogenesis; topogenic sequences which regulate the integration and topology of membrane proteins in the endoplasmic reticulum membrane; what can glycosylation mapping tell us about membrane protein assembly and structure?; molecular determinants of intracellular processing of human pro-tumour necrosis factor; protein degradation in the endoplasmic reticulum; SPR-like particle and its receptor system of "bacillus subtilis" - identification and characterization; identification and functional characterization of nascent-polypeptide-associated complex; the SecY complex and the FtsH complex in the "E.coli" plasma membrane; topology inversion of SecG, a membrane factor of "E.coli" protein translocase; assembly of thylakoid membrane proteins in chloroplasts. Part 3 Structure and function: studies on the glut family transporters - use of the yeast expression system; internal water molecules in the functional process of bacteriorhodopsin and rhodopsin; regulation of erythrocyte potassium transport by cell volume and protein phosphorylation; membrane translocation of cytosolic factors in activation of the phagocyte NADPF oxidase - role of protein-protein interactions; three-dimensional cryo-electron microscopy of calcium ion pump; the aquaporin family of water channel proteins; aquaporin 2 vasopressin water channel; the Na+/K+-ATPase - molecular dissection of functional domains; significance of the beta-subunit in the functional expression of Na+/K+-ATPase; gastric H+, K+-ATPase - its functional expression and site-directed mutagenesis; structure and N-glycosylation of human band 3, the erythrocyte anion exchanger; phenotypic diversity of band 3 mutations; the carboxy terminal region of the human AE1 (erythrocyte band 3 protein); the expression of the erythrocyte anion transporter (band 3, AE1); studies on the pH dependence of band 3-mediated anion transport, facts and speculations.
